Differential cellular immune response of Galleria mellonella to Actinobacillus pleuropneumoniae

dc.creatorBlanco, Luis Andrés Arteaga
dc.creatorCrispim, Josicelli Souza
dc.creatorFernandes, Kenner Morais
dc.creatorOliveira, Leandro Licursi de
dc.creatorPereira, Monalessa Fábia
dc.creatorBazzolli, Denise Mara Soares
dc.creatorMartins, Gustavo Ferreira
dc.date2018-03-22T12:05:56Z
dc.date2018-03-22T12:05:56Z
dc.date2017-07-08
dc.date.accessioned2023-09-27T20:53:06Z
dc.date.available2023-09-27T20:53:06Z
dc.identifier14320878
dc.identifierhttps://doi.org/10.1007/s00441-017-2653-5
dc.identifierhttp://www.locus.ufv.br/handle/123456789/18386
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/8950112
dc.descriptionIn the present work, we have investigate the cellular immune response of Galleria mellonella larvae against three strains of the gram-negative bacterium Actinobacillus pleuropneumoniae: low-virulence (780), high-virulence (1022) and the serotype 8 reference strain (R8). Prohemocytes, plasmatocytes, granulocytes, oenocytoids and spherulocytes were distinguished according to their size and morphology, their molecular markers and dye-staining properties and their role in the immune response. Total hemocyte count, differential hemocyte count, lysosome activity, autophagic response, cell viability and caspase-3 activation were determined in circulating hemocytes of naive and infected larvae. The presence of the autophagosome protein LC3 A/B within the circulating hemocytes of G. mellonella was dependent on and related to the infecting A. pleuropneumoniae strain and duration of infection. Hemocytes treated with the high-virulence strain expressed higher levels of LC3 A/B, whereas treatment with the low-virulence strain induced lower expression levels of this protein in the cells. Moreover, our results showed that apoptosis in circulating hemocytes of G. mellonella larvae after exposure to virulent bacterial strains occurred simultaneously with excessive cell death response induced by stress and subsequent caspase-3 activation.
dc.formatpdf
dc.formatapplication/pdf
dc.languageeng
dc.publisherCell and Tissue Research
dc.relationv. 370, Issue 1, p. 153–168, October 2017
dc.rightsSpringer Berlin Heidelberg
dc.subjectApoptosis
dc.subjectAutophagy
dc.subjectImmune response
dc.subjectActinobacillus pleuropneumoniae
dc.subjectGalleria mellonella
dc.titleDifferential cellular immune response of Galleria mellonella to Actinobacillus pleuropneumoniae
dc.typeArtigo


Este ítem pertenece a la siguiente institución