A hydrogel scaffold that maintains viability and supports differentiation of dental pulp stem cells

dc.contributorUniversidade Estadual Paulista (UNESP)
dc.creatorCavalcanti, Bruno N.
dc.creatorZeitlin, Benjamin D.
dc.creatorNör, Jacques E.
dc.date2014-05-27T11:27:28Z
dc.date2016-10-25T18:40:54Z
dc.date2014-05-27T11:27:28Z
dc.date2016-10-25T18:40:54Z
dc.date2013-01-01
dc.date.accessioned2017-04-06T02:07:07Z
dc.date.available2017-04-06T02:07:07Z
dc.identifierDental Materials, v. 29, n. 1, p. 97-102, 2013.
dc.identifier0109-5641
dc.identifierhttp://hdl.handle.net/11449/74169
dc.identifierhttp://acervodigital.unesp.br/handle/11449/74169
dc.identifier10.1016/j.dental.2012.08.002
dc.identifierWOS:000312269000008
dc.identifier2-s2.0-84871014475
dc.identifierhttp://dx.doi.org/10.1016/j.dental.2012.08.002
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/894942
dc.descriptionObjectives: The clinical translation of stem cell-based Regenerative Endodontics demands further development of suitable injectable scaffolds. Puramatrix™ is a defined, self-assembling peptide hydrogel which instantaneously polymerizes under normal physiological conditions. Here, we assessed the compatibility of Puramatrix™ with dental pulp stem cell (DPSC) growth and differentiation. Methods: DPSC cells were grown in 0.05-0.25% Puramatrix™. Cell viability was measured colorimetrically using the WST-1 assay. Cell morphology was observed in 3D modeling using confocal microscopy. In addition, we used the human tooth slice model with Puramatrix™ to verify DPSC differentiation into odontoblast-like cells, as measured by expression of DSPP and DMP-1. Results: DPSC survived and proliferated in Puramatrix™ for at least three weeks in culture. Confocal microscopy revealed that cells seeded in Puramatrix™ presented morphological features of healthy cells, and some cells exhibited cytoplasmic elongations. Notably, after 21 days in tooth slices containing Puramatrix™, DPSC cells expressed DMP-1 and DSPP, putative markers of odontoblastic differentiation. Significance: Collectively, these data suggest that self-assembling peptide hydrogels might be useful injectable scaffolds for stem cell-based Regenerative Endodontics. © 2012 Academy of Dental Materials.
dc.languageeng
dc.relationDental Materials
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.subjectDental pulp
dc.subjectHydrogel
dc.subjectOdontoblast
dc.subjectRegenerative Endodontics
dc.subjectStem cells
dc.subjectTissue engineering
dc.subject3-d modeling
dc.subjectCell morphology
dc.subjectCell viability
dc.subjectCell-based
dc.subjectClinical translation
dc.subjectHuman teeths
dc.subjectHydrogel scaffolds
dc.subjectInjectable scaffolds
dc.subjectMorphological features
dc.subjectPhysiological condition
dc.subjectPuraMatrix
dc.subjectSelf-assembling peptides
dc.subjectCell culture
dc.subjectConfocal microscopy
dc.subjectDentistry
dc.subjectHydrogels
dc.subjectScaffolds (biology)
dc.titleA hydrogel scaffold that maintains viability and supports differentiation of dental pulp stem cells
dc.typeOtro


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