An integrated cytogenetic, flow and image cytometry procedure used to measure the DNA content of Zea mays A and B chromosomes

dc.creatorRosado, Tatiana Barbosa
dc.creatorClarindo, Wellington Ronildo
dc.creatorCarvalho, Carlos Roberto
dc.date2018-10-19T11:32:01Z
dc.date2018-10-19T11:32:01Z
dc.date2008-10-31
dc.date.accessioned2023-09-27T20:44:53Z
dc.date.available2023-09-27T20:44:53Z
dc.identifier01689452
dc.identifierhttps://doi.org/10.1016/j.plantsci.2008.10.007
dc.identifierhttp://www.locus.ufv.br/handle/123456789/22358
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/8946993
dc.descriptionThe DNA content of some Zea mays chromosomes has been measured by flow karyotyping because similar chromosomes could not been discriminated. This technique requires suspensions with a high amount of intact chromosomes and protocols that provide enough resolution to quantify the DNA content of individual chromosomes. In order to measure the DNA content of A and B chromosomes of Z. mays ‘Black Mexican Sweet Corn’, we associated cytogenetic, flow and image cytometry tools. The cytogenetic methodology provided slides with A and B chromosomes adequate for karyogram assembly. The flow cytometry histograms exhibited distinct G0/G1 and G2 peaks for ‘Black Mexican Sweet Corn’ with (sample) and without (standard) B chromosomes. As a result, the nuclear DNA content of five plants showing B chromosomes was quantified. The nuclear genome size value was proportionally distributed according to the integrated optical density value, which was measured for individual chromosomes by image cytometry. Then, the DNA content of each A and B chromosome was calculated in picograms and base pairs. Based on qualitative (cytogenetic) and quantitative (flow and image cytometry) analyses, it was possible to discriminate and characterize the A and B chromosomes of ‘Black Mexican Sweet Corn’.
dc.formatpdf
dc.formatapplication/pdf
dc.languageeng
dc.publisherPlant Science
dc.relationv. 176, n. 1, p. 154- 158, jan. 2009
dc.rightsElsevier Ireland Ltd.
dc.subjectZea mays
dc.subjectImage cytometry
dc.subjectFlow cytometry
dc.subjectCytogenetic
dc.subjectB chromosomes
dc.subjectChromosomal DNA content
dc.titleAn integrated cytogenetic, flow and image cytometry procedure used to measure the DNA content of Zea mays A and B chromosomes
dc.typeArtigo


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