A naturally occurring recombinant DNA-A of a typical bipartite begomovirus does not require the cognate DNA-B to infect Nicotiana benthamiana systemically

dc.creatorGalvao, Rafaelo M.
dc.creatorMariano, Andrea C.
dc.creatorLuz, Dirce F.
dc.creatorAlfenas, Poliane F.
dc.creatorAndrade, Eduardo C.
dc.creatorZerbini, Francisco M.
dc.creatorAlmeida, Marcia R.
dc.creatorFontes, Elizabeth P. B.
dc.date2018-04-19T18:14:17Z
dc.date2018-04-19T18:14:17Z
dc.date2003-03-01
dc.date.accessioned2023-09-27T20:39:28Z
dc.date.available2023-09-27T20:39:28Z
dc.identifier00221317
dc.identifierhttp://dx.doi.org/10.1099/vir.0.18783-0
dc.identifierhttp://www.locus.ufv.br/handle/123456789/18896
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/8946028
dc.descriptionSpecies of the genus Begomovirus (family Geminiviridae) found in the western hemisphere typically have a bipartite genome that consists of two 26 kb DNA genomic components, DNA-A and DNA-B. We have identified and cloned genomic components of a new tomato-infecting begomovirus from Brazil, for which the name Tomato crinkle leaf yellows virus (TCrLYV) is proposed, and a DNA-A variant of Tomato chlorotic mottle virus (ToCMV-[MG-Bt1]). Sequence analysis revealed that TCrLYV was most closely related to ToCMV, although it was sufficiently divergent to be considered a distinct virus species. Furthermore, these closely related viruses induce distinguishable symptoms in tomato plants. With respect to ToCMV-[MG-Bt1] DNA-A, evidence is presented that suggests a recombinant origin. It possesses a hybrid genome on which the replication compatible module (AC1 and replication origin) was probably donated by ToCMV- [BA-Se1] and the remaining sequences appear to have originated from Tomato rugose mosaic virus (ToRMV). Despite the high degree of sequence conservation with its predecessors, ToCMV-[MG- Bt1] differs significantly in its biological properties. Although ToCMV-[MG-Bt1] DNA-A did not infect tomato plants, it systemically infected Nicotiana benthamiana, induced symptoms of mottling and accumulated viral DNA in the apical leaves in the absence of a cognate DNA-B. The modular rearrangement that resulted in ToCMV-[MG-Bt1] DNA-A may have provided this virus with a more aggressive nature. Our results further support the notion that interspecies recombination may play a significant role in geminivirus diversity and their emergence as agriculturally important pathogens.
dc.formatpdf
dc.formatapplication/pdf
dc.languageeng
dc.publisherJournal of General Virology
dc.relationv. 84, p. 715–726, march 2003
dc.rightsSGM
dc.subjectDNA-A
dc.subjectDNA-B
dc.subjectNicotiana benthamiana
dc.titleA naturally occurring recombinant DNA-A of a typical bipartite begomovirus does not require the cognate DNA-B to infect Nicotiana benthamiana systemically
dc.typeArtigo


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