Electrogravimetric Analysis by Quartz-Crystal Microbalance on the Consumption of the Neurotransmitter Acetylcholine by Acetylcholinesterase

dc.contributorUniversidade Estadual Paulista (UNESP)
dc.creatorBueno, Paulo Roberto
dc.creatorGonçalves, Luís M.
dc.creatordos Santos, Fernanda C.
dc.creatordos Santos, Marcio L.
dc.creatorBarros, Aquiles A.
dc.creatorFaria, Ronaldo C.
dc.date2014-05-27T11:27:17Z
dc.date2016-10-25T18:39:59Z
dc.date2014-05-27T11:27:17Z
dc.date2016-10-25T18:39:59Z
dc.date2012-12-01
dc.date.accessioned2017-04-06T02:03:54Z
dc.date.available2017-04-06T02:03:54Z
dc.identifierAnalytical Letters, v. 46, n. 2, p. 258-265, 2012.
dc.identifier0003-2719
dc.identifier1532-236X
dc.identifierhttp://hdl.handle.net/11449/73799
dc.identifierhttp://acervodigital.unesp.br/handle/11449/73799
dc.identifier10.1080/00032719.2012.713065
dc.identifierWOS:000312984400004
dc.identifier2-s2.0-84871859504
dc.identifierhttp://dx.doi.org/10.1080/00032719.2012.713065
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/894584
dc.descriptionElectrogravimetric analysis was performed on the consumption of the neurotransmitter Acetylcholine (ACh) by Acetylcholinesterase (AChE) in situ and in real time. Michaelis-Menten assumption was achieved by using an enzyme micro-reactor in which the total enzyme was anchored in a quartz crystal microbalance chip (QCM-chip) with a strategically engineered self-assembled monolayer (SAM) of alkanethiols, which can prevent diffusion-controlled or spatially restricted kinetics. The real-time frequency changes indicated the rate of the products formation from enzymatic reaction. The QCM-chip was tested showing that it could demonstrate AChE inhibition by physostigmine. © 2013 Copyright Taylor and Francis Group, LLC.
dc.languageeng
dc.relationAnalytical Letters
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.subjectAcetyl cholinesterase
dc.subjectAcetylcholine
dc.subjectNeurotransmitter
dc.subjectPhysostigmine
dc.subjectQuartz-crystal microbalance
dc.titleElectrogravimetric Analysis by Quartz-Crystal Microbalance on the Consumption of the Neurotransmitter Acetylcholine by Acetylcholinesterase
dc.typeOtro


Este ítem pertenece a la siguiente institución