Interference of raw milk autochthonous microbiota on the performance of conventional methodologies for Listeria monocytogenes and Salmonella spp. detection

dc.creatorNero, Luís Augusto
dc.creatorMattos, Marcos Rodriguesde
dc.creatorBarros, Márcia de Aguiar Ferreira
dc.creatorBeloti, Vanerli
dc.creatorFranco, Bernadette Dora Gombossy de Melo
dc.date2017-11-29T15:42:47Z
dc.date2017-11-29T15:42:47Z
dc.date2009-09-29
dc.date.accessioned2023-09-27T20:38:44Z
dc.date.available2023-09-27T20:38:44Z
dc.identifier23284137
dc.identifierhttps://doi.org/10.1016/j.micres.2007.04.003
dc.identifierhttp://www.locus.ufv.br/handle/123456789/13981
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/8945723
dc.descriptionPathogen detection in foods by reliable methodologies is very important to guarantee microbiological safety. However, peculiar characteristics of certain foods, such as autochthonous microbiota, can directly influence pathogen development and detection. With the objective of verifying the performance of the official analytical methodologies for the isolation of Listeria monocytogenes and Salmonella in milk, different concentrations of these pathogens were inoculated in raw milk treatments with different levels of mesophilic aerobes, and then submitted to the traditional isolation procedures for the inoculated pathogens. Listeria monocytogenes was inoculated at the range of 0.2–5.2 log CFU/mL in treatments with 1.8–8.2 log CFU/mL. Salmonella Enteritidis was inoculated at 0.9–3.9 log CFU/mL in treatments with 3.0–8.2 log CFU/mL. The results indicated that recovery was not possible or was more difficult in the treatments with high counts of mesophilic aerobes and low levels of the pathogens, indicating interference of raw milk autochthonous microbiota.
dc.formatpdf
dc.formatapplication/pdf
dc.languageeng
dc.publisherMicrobiological Research
dc.relationVolume 164, Issue 5, Pages 529-535, September 2009
dc.rightsOpen Access
dc.subjectAutochthonous microbiota
dc.subjectMilk
dc.subjectPathogen detection
dc.subjectListeria monocytogenes
dc.subjectSalmonella spp
dc.titleInterference of raw milk autochthonous microbiota on the performance of conventional methodologies for Listeria monocytogenes and Salmonella spp. detection
dc.typeArtigo


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