Boto, a class II transposon in Moniliophthora perniciosa, is the first representative of the PIF/ Harbinger superfamily in a phytopathogenic fungus

dc.creatorPereira, Jorge Fernando
dc.creatorAlmeida, Ana Paula Morais Martins
dc.creatorCota, Júnio
dc.creatorPamphile, João Alencar
dc.creatorSilva, Gilvan Ferreira da
dc.creatorAraújo, Elza Fernandes de
dc.creatorGramacho, Karina Peres
dc.creatorBrommonschenkel, Sérgio Hermı́nio
dc.creatorPereira, Gonçalo Amarante Guimarães
dc.creatorQueiroz, Marisa Vieira de
dc.date2018-05-16T12:10:59Z
dc.date2018-05-16T12:10:59Z
dc.date2012-10-24
dc.date.accessioned2023-09-27T20:37:47Z
dc.date.available2023-09-27T20:37:47Z
dc.identifier14652080
dc.identifierhttps://doi.org/10.1099/mic.0.062901-0
dc.identifierhttp://www.locus.ufv.br/handle/123456789/19604
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/8945329
dc.descriptionBoto, a class II transposable element, was characterized in the Moniliophthora perniciosa genome. The Boto transposase is highly similar to plant PIF-like transposases that belong to the newest class II superfamily known as PIF/Harbinger. Although Boto shares characteristics with PIF-like elements, other characteristics, such as the transposase intron position, the position and direction of the second ORF, and the footprint, indicate that Boto belongs to a novel family of the PIF/Harbinger superfamily. Southern blot analyses detected 6–12 copies of Boto in C-biotype isolates and a ubiquitous presence among the C- and S-biotypes, as well as a separation in the C-biotype isolates from Bahia State in Brazil in at least two genotypic groups, and a new insertion in the genome of a C-biotype isolate maintained in the laboratory for 6 years. In addition to PCR amplification from a specific insertion site, changes in the Boto hybridization profile after the M. perniciosa sexual cycle and detection of Boto transcripts gave further evidence of Boto activity. As an active family in the genome of M. perniciosa, Boto elements may contribute to genetic variability in this homothallic fungus. This is the first report of a PIF/Harbinger transposon in the genome of a phytopathogenic fungus.
dc.formatpdf
dc.formatapplication/pdf
dc.languageeng
dc.publisherMicrobiology
dc.relationv. 159, p. 112-125, January 2013
dc.rightsSGM
dc.subjectMoniliophthora perniciosa
dc.subjectPIF/Harbinger
dc.titleBoto, a class II transposon in Moniliophthora perniciosa, is the first representative of the PIF/ Harbinger superfamily in a phytopathogenic fungus
dc.typeArtigo


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