Adapted colorimetric method for measurement of feline urinary glycosaminoglycans

dc.contributorUniversidade Estadual Paulista (UNESP)
dc.creatorPereira, Marcy Lancia
dc.creatorPizauro Jr., João Martins
dc.creatorCarvalho, Marileda Bonafim
dc.date2014-05-27T11:27:03Z
dc.date2016-10-25T18:38:38Z
dc.date2014-05-27T11:27:03Z
dc.date2016-10-25T18:38:38Z
dc.date2012-10-01
dc.date.accessioned2017-04-06T02:01:34Z
dc.date.available2017-04-06T02:01:34Z
dc.identifierComparative Clinical Pathology, p. 1-4.
dc.identifier1618-5641
dc.identifier1618-565X
dc.identifierhttp://hdl.handle.net/11449/73604
dc.identifierhttp://acervodigital.unesp.br/handle/11449/73604
dc.identifier10.1007/s00580-012-1616-1
dc.identifier2-s2.0-84866674392
dc.identifierhttp://dx.doi.org/10.1007/s00580-012-1616-1
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/894402
dc.descriptionSeveral methods have been employed to quantify urinary glycosaminoglycans (GAGs), such as chromatography associated with electrophoresis and colorimetric methods, cheaper and faster ones, which employ mainly azure A and B, alcian blue, and dimethylmethylene blue (DMB). The purpose of this study was to standardize a reproducible and cheap method to measure total urinary GAGs in feline urine. Two colorimetric methods based on DMB were tested with chondroitin sulfate C as standard. Urine samples were obtained from 12 healthy cats and some modifications were made for the chosen method to be adequate. The modified technique using DMB acetate buffer carried out in this study can be used to measure feline urinary GAGs. © 2012 Springer-Verlag London.
dc.languageeng
dc.relationComparative Clinical Pathology
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.subjectCat
dc.subjectDMB
dc.subjectGlycosaminoglycan
dc.subjectQuantification
dc.subjectUrine
dc.titleAdapted colorimetric method for measurement of feline urinary glycosaminoglycans
dc.typeOtro


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