Use of reverse transcriptase polymerase chain reaction (RT-PCR) in molecular screening of Newcastle disease virus in poultry and free-living bird populations

dc.contributorUniversidade Estadual Paulista (UNESP)
dc.creatorde Oliveira Torres Carrasco, Adriano
dc.creatorRodrigues, Juliana Nogueira Martins
dc.creatorSeki, Meire Christina
dc.creatorde Moraes, Fabricio Edgar
dc.creatorSilva, Jaqueline Raymondi
dc.creatorDurigon, Edison Luis
dc.creatorPinto, Aramis Augusto
dc.date2014-05-27T11:27:02Z
dc.date2016-10-25T18:38:33Z
dc.date2014-05-27T11:27:02Z
dc.date2016-10-25T18:38:33Z
dc.date2012-09-17
dc.date.accessioned2017-04-06T02:01:19Z
dc.date.available2017-04-06T02:01:19Z
dc.identifierTropical Animal Health and Production, v. 45, n. 1, p. 569-576, 2012.
dc.identifier0049-4747
dc.identifier1573-7438
dc.identifierhttp://hdl.handle.net/11449/73577
dc.identifierhttp://acervodigital.unesp.br/handle/11449/73577
dc.identifier10.1007/s11250-012-0261-7
dc.identifierWOS:000314046100031
dc.identifier2-s2.0-84872827481
dc.identifierhttp://dx.doi.org/10.1007/s11250-012-0261-7
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/894378
dc.descriptionThe aim of this study was to evaluate a simple molecular method of reverse transcriptase polymerase chain reaction (RT-PCR) to differentiate Newcastle disease virus strains according to their pathogenicity, in order to use it in molecular screening of Newcastle disease virus in poultry and free-living bird populations. Specific primers were developed to differentiate LaSota-LS-(vaccine strain) and Sao Joao do Meriti-SJM-strain (highly pathogenic strain). Chickens and pigeons were experimentally vaccinated/infected for an in vivo study to determine virus shedding in feces. Validation of sensitivity and specificity of the primers (SJM and LS) by experimental models used in the present study and results obtained in the molecular analysis of the primers by BLAST made it possible to generalize results. The development of primers that differentiate the level of pathogenicity of NDV stains is very important, mainly in countries where real-time RT-PCR is still not used as a routine test. These primers were able to determine the presence of the agent and to differentiate it according to its pathogenicity. © 2012 Springer Science+Business Media B.V.
dc.languageeng
dc.relationTropical Animal Health and Production
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.subjectDiagnosis
dc.subjectExperimental infection
dc.subjectNewcastle disease virus
dc.subjectPoultry
dc.subjectAves
dc.subjectColumba
dc.subjectGallus gallus
dc.subjectprimer DNA
dc.subjectanimal
dc.subjectanimal disease
dc.subjectbird disease
dc.subjectchicken
dc.subjectevaluation
dc.subjectfeces
dc.subjectgenetics
dc.subjectisolation and purification
dc.subjectmethodology
dc.subjectNewcastle disease
dc.subjectNewcastle disease paramyxovirus
dc.subjectpigeons and doves
dc.subjectreverse transcription polymerase chain reaction
dc.subjectsensitivity and specificity
dc.subjectvirology
dc.subjectvirus shedding
dc.subjectAnimals
dc.subjectChickens
dc.subjectColumbidae
dc.subjectDNA Primers
dc.subjectFeces
dc.subjectNewcastle Disease
dc.subjectPoultry Diseases
dc.subjectReverse Transcriptase Polymerase Chain Reaction
dc.subjectSensitivity and Specificity
dc.subjectVirus Shedding
dc.titleUse of reverse transcriptase polymerase chain reaction (RT-PCR) in molecular screening of Newcastle disease virus in poultry and free-living bird populations
dc.typeOtro


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