Physical factors affecting plasmid DNA compaction in stearylamine-containing nanoemulsions intended for gene delivery

dc.contributorUniversidade Estadual Paulista (UNESP)
dc.creatorSilva, André Leandro
dc.creatorAlexandrino Jr., Francisco
dc.creatorVerissimo, Lourena Mafra
dc.creatorAgnez-Lima, Lucymara Fassarella
dc.creatorEgito, Lucila Carmem Monte
dc.creatorOliveira, Anselmo Gomes de
dc.creatordo Egito, Eryvaldo Socrates Tabosa
dc.date2014-05-27T11:26:50Z
dc.date2016-10-25T18:37:23Z
dc.date2014-05-27T11:26:50Z
dc.date2016-10-25T18:37:23Z
dc.date2012-06-13
dc.date.accessioned2017-04-06T01:59:10Z
dc.date.available2017-04-06T01:59:10Z
dc.identifierPharmaceuticals, v. 5, n. 6, p. 643-654, 2012.
dc.identifier1424-8247
dc.identifierhttp://hdl.handle.net/11449/73378
dc.identifierhttp://acervodigital.unesp.br/handle/11449/73378
dc.identifier10.3390/ph5060643
dc.identifier2-s2.0-84863632967.pdf
dc.identifier2-s2.0-84863632967
dc.identifierhttp://dx.doi.org/10.3390/ph5060643
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/894187
dc.descriptionCationic lipids have been used in the development of non-viral gene delivery systems as lipoplexes. Stearylamine, a cationic lipid that presents a primary amine group when in solution, is able to compact genetic material by electrostatic interactions. In dispersed systems such as nanoemulsions this lipid anchors on the oil/water interface confering a positive charge to them. The aim of this work was to evaluate factors that influence DNA compaction in cationic nanoemulsions containing stearylamine. The influence of the stearylamine incorporation phase (water or oil), time of complexation, and different incubation temperatures were studied. The complexation rate was assessed by electrophoresis migration on agarose gel 0.7%, and nanoemulsion and lipoplex characterization was done by Dynamic Light Scattering (DLS). The results demonstrate that the best DNA compaction process occurs after 120 min of complexation, at low temperature (4 ± 1 °C), and after incorporation of the cationic lipid into the aqueous phase. Although the zeta potential of lipoplexes was lower than the results found for basic nanoemulsions, the granulometry did not change. Moreover, it was demonstrated that lipoplexes are suitable vehicles for gene delivery. © 2012 by the authors; licensee MDPI, Basel, Switzerland.
dc.languageeng
dc.relationPharmaceuticals
dc.rightsinfo:eu-repo/semantics/openAccess
dc.subjectCationic lipid nanoemulsions
dc.subjectGene therapy
dc.subjectStearylamine
dc.subjectlipid
dc.subjectoctadecylamine
dc.subjectplasmid DNA
dc.subjectcomplex formation
dc.subjectelectrophoresis
dc.subjectincubation time
dc.subjectlight scattering
dc.subjectlow temperature
dc.subjectnanoemulsion
dc.subjectnonviral gene delivery system
dc.subjectparticle size
dc.subjecttemperature sensitivity
dc.subjectzeta potential
dc.titlePhysical factors affecting plasmid DNA compaction in stearylamine-containing nanoemulsions intended for gene delivery
dc.typeOtro


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