Effect of bleaching on sound enamel and with early artificial caries lesions using confocal laser microscopy

dc.contributorUniversidade Estadual Paulista (UNESP)
dc.creatorBerger, Sandrine Bittencourt
dc.creatorPavan, Sabrina
dc.creatorSantos, Paulo Henrique dos
dc.creatorGiannini, Marcelo
dc.creatorBedran-Russo, Ana Karina B.
dc.date2014-05-27T11:26:24Z
dc.date2016-10-25T18:36:46Z
dc.date2014-05-27T11:26:24Z
dc.date2016-10-25T18:36:46Z
dc.date2012-03-01
dc.date.accessioned2017-04-06T01:57:32Z
dc.date.available2017-04-06T01:57:32Z
dc.identifierBrazilian Dental Journal, v. 23, n. 2, p. 110-115, 2012.
dc.identifier0103-6440
dc.identifier1806-4760
dc.identifierhttp://hdl.handle.net/11449/73215
dc.identifierhttp://acervodigital.unesp.br/handle/11449/73215
dc.identifier10.1590/S0103-64402012000200004
dc.identifierS0103-64402012000200004
dc.identifier2-s2.0-84862598159.pdf
dc.identifier2-s2.0-84862598159
dc.identifierhttp://dx.doi.org/10.1590/S0103-64402012000200004
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/894035
dc.descriptionThe aim of this study was to evaluate effect of bleaching agents on sound enamel (SE) and enamel with early artificial caries lesions (CL) using confocal laser scanning microscopy (CLSM). Eighty blocks (4 × 5 × 5 mm) of bovine enamel were used and half of them were submitted to a pH cycling model to induce CL. Eight experimental groups were obtained from the treatments and mineralization level of the enamel (SE or CL) (n=10). SE groups: G1 - unbleached (control); G2 - 4% hydrogen peroxide (4 HP); G3 - 4 HP containing 0.05% Ca (Ca); G4 - 7.5% hydrogen peroxide (7.5 HP) containing amorphous calcium phosphate (ACP). CL groups: G5 - unbleached; G6 - 4 HP; G7 - 4 HP containing Ca; G8 - 7.5 HP ACP. G2, G3, G6, G7 were treated with the bleaching agents for 8 h/day during 14 days, while G4 and G8 were exposed to the bleaching agents for 30 min twice a day during 14 days. The enamel blocks were stained with 0.1 mM rhodamine B solution and the demineralization was quantified using fluorescence intensity detected by CLSM. Data were analyzed using ANOVA and Fisher's tests (α=0.05). For the SE groups, the bleaching treatments increased significantly the demineralization area when compared with the unbleached group. In the CL groups, no statistically significant difference was observed (p>0.05). The addition of ACP or Ca in the composition of the whitening products did not overcome the effects caused by bleaching treatments on SE and neither was able to promote remineralization of CL.
dc.languageeng
dc.relationBrazilian Dental Journal
dc.rightsinfo:eu-repo/semantics/openAccess
dc.subjectBovine enamel
dc.subjectConfocal laser scanning microscopy
dc.subjectWhitening
dc.subjectamorphous calcium phosphate
dc.subjectcalcium phosphate
dc.subjecthydrogen peroxide
dc.subjecttooth bleaching agent
dc.subjectanalysis of variance
dc.subjectanimal
dc.subjectcattle
dc.subjectchemically induced disorder
dc.subjectconfocal microscopy
dc.subjectdental caries
dc.subjectdental procedure
dc.subjectdrug effect
dc.subjectenamel
dc.subjecthardness
dc.subjectmethodology
dc.subjectpathophysiology
dc.subjecttooth disease
dc.subjectAnalysis of Variance
dc.subjectAnimals
dc.subjectCalcium Phosphates
dc.subjectCattle
dc.subjectDental Caries
dc.subjectDental Enamel
dc.subjectHardness
dc.subjectHydrogen Peroxide
dc.subjectMicroscopy, Confocal
dc.subjectTooth Bleaching
dc.subjectTooth Bleaching Agents
dc.subjectTooth Demineralization
dc.subjectTooth Remineralization
dc.titleEffect of bleaching on sound enamel and with early artificial caries lesions using confocal laser microscopy
dc.typeOtro


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