Baccharis dracunculifolia, the main source of green propolis, exhibits potent antioxidant activity and prevents oxidative mitochondrial damage

dc.contributorUniversidade Estadual Paulista (UNESP)
dc.creatorGuimarães, Natalia S.S.
dc.creatorMello, Joyce C.
dc.creatorPaiva, Juliana S.
dc.creatorBueno, Paula C.P.
dc.creatorBerretta, Andresa A.
dc.creatorTorquato, Ricardo J.
dc.creatorNantes, Iseli L.
dc.creatorRodrigues, Tiago
dc.date2014-05-27T11:26:23Z
dc.date2016-10-25T18:36:37Z
dc.date2014-05-27T11:26:23Z
dc.date2016-10-25T18:36:37Z
dc.date2012-03-01
dc.date.accessioned2017-04-06T01:57:24Z
dc.date.available2017-04-06T01:57:24Z
dc.identifierFood and Chemical Toxicology, v. 50, n. 3-4, p. 1091-1097, 2012.
dc.identifier0278-6915
dc.identifier1873-6351
dc.identifierhttp://hdl.handle.net/11449/73200
dc.identifierhttp://acervodigital.unesp.br/handle/11449/73200
dc.identifier10.1016/j.fct.2011.11.014
dc.identifier2-s2.0-84856478927.pdf
dc.identifier2-s2.0-84856478927
dc.identifierhttp://dx.doi.org/10.1016/j.fct.2011.11.014
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/894020
dc.descriptionBaccharis dracunculifolia DC (Asteraceae) is the main botanical source used by honeybees to produce Brazilian green propolis whose hepatoprotective properties have been already described. In this work we investigated the protective effects of the glycolic extract of B. dracunculifolia (GEBd) against oxidative stress in isolated rat liver mitochondria (RLM). The GEBd was prepared by fractionated percolation using propylene glycol as solvent. The total phenols and flavonoids, which are substances with recognized antioxidant action, were quantified in GEBd and the phytochemical analysis was carried out by HPLC. GEBd exhibited significant scavenger activity towards DPPH radicals and superoxide anions in a concentration-dependent manner, and also a Fe 2+ chelating activity. GEBd decreased the basal H 2O 2 generation and the Fe 2+- or t-BuOOH-induced ROS production in isolated mitochondria. Lipid oxidation of mitochondrial membranes, protein thiol groups and GSH oxidation were also prevented by GEBd. This shows that B. dracunculifolia exhibit potent antioxidant activity protecting liver mitochondria against oxidative damage and such action probably contribute to the antioxidant and hepatoprotective effects of green propolis. © 2011 Elsevier Ltd.
dc.languageeng
dc.relationFood and Chemical Toxicology
dc.rightsinfo:eu-repo/semantics/openAccess
dc.subjectAntioxidant
dc.subjectBaccharis dracunculifolia
dc.subjectFree radicals
dc.subjectHepatoprotection
dc.subjectMitochondria
dc.subjectOxidative stress
dc.subject1,1 diphenyl 2 picrylhydrazyl
dc.subjectantioxidant
dc.subjectBaccharis dracunculifolia extract
dc.subjectferrous ion
dc.subjectflavonoid
dc.subjectglutathione
dc.subjecthydrogen peroxide
dc.subjectphenol
dc.subjectplant extract
dc.subjectpropolis
dc.subjectpropylene glycol
dc.subjectscavenger
dc.subjectsuperoxide
dc.subjectthiol group
dc.subjectunclassified drug
dc.subjectanimal tissue
dc.subjectantioxidant activity
dc.subjectBaccharis
dc.subjectbaccharis dracunculifolia
dc.subjectcell protection
dc.subjectconcentration response
dc.subjectcontrolled study
dc.subjectdown regulation
dc.subjectdrug mechanism
dc.subjectdrug potency
dc.subjecthigh performance liquid chromatography
dc.subjectiron chelation
dc.subjectlipid oxidation
dc.subjectliver mitochondrion
dc.subjectliver protection
dc.subjectmale
dc.subjectmitochondrial membrane
dc.subjectnonhuman
dc.subjectoxidative stress
dc.subjectphytochemistry
dc.subjectrat
dc.subjectAnimals
dc.subjectAntioxidants
dc.subjectChromatography, High Pressure Liquid
dc.subjectGlutathione
dc.subjectLipids
dc.subjectMale
dc.subjectMitochondria, Liver
dc.subjectOxidation-Reduction
dc.subjectOxidative Stress
dc.subjectPropolis
dc.subjectRats
dc.subjectRats, Wistar
dc.subjectSpectrophotometry, Ultraviolet
dc.subjectAsteraceae
dc.subjectRattus
dc.titleBaccharis dracunculifolia, the main source of green propolis, exhibits potent antioxidant activity and prevents oxidative mitochondrial damage
dc.typeOtro


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