CD63 e CD123 expressão, autoanticorpos IgG e acurácia do teste do soro autólogo em pacientes com urticária crônica

dc.contributorUniversidade Estadual Paulista (UNESP)
dc.creatorCalamita, Zamir
dc.creatorAntunes, Roseli Nunes da Silveira
dc.creatorAlmeida Filho, Odilon Marques de
dc.creatorBaleotti Júnior, Wilson
dc.creatorCalamita, Andrea Bronhara Pelá
dc.creatorFukasawa, Josianne Thomazini
dc.creatorCavaretto, Débora de Aguiar
dc.date2014-05-27T11:26:23Z
dc.date2016-10-25T18:36:35Z
dc.date2014-05-27T11:26:23Z
dc.date2016-10-25T18:36:35Z
dc.date2012-02-01
dc.date.accessioned2017-04-06T01:57:14Z
dc.date.available2017-04-06T01:57:14Z
dc.identifierJornal Brasileiro de Patologia e Medicina Laboratorial, v. 48, n. 1, p. 21-28, 2012.
dc.identifier1676-2444
dc.identifier1678-4774
dc.identifierhttp://hdl.handle.net/11449/73180
dc.identifierhttp://acervodigital.unesp.br/handle/11449/73180
dc.identifier10.1590/S1676-2444201200010000
dc.identifierS1676-2444201200010000
dc.identifier2-s2.0-84858650086.pdf
dc.identifier2-s2.0-84858650086
dc.identifierhttp://dx.doi.org/10.1590/S1676-2444201200010000
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/894001
dc.descriptionIntroduction: The autologous serum skin test (ASST) may suggest an autoimmune etiology in chronic urticaria (CU). A new laboratory technique called basophil activation test (BAT) has been currently employed for its diagnosis. Objective: To analyze ASST in relation to BAT as well as to evaluate interleukin 3 (IL3) receptors (CD123) and non-specific immunoglobulin G (IgG) autoantibodies bound to basophils in patients with chronic urticaria. Methods: We studied 33 adults with CU and mean age of 42.5 + 14 years. After stimulation by serum from patients with CU, CD63 expression on basophils from one atopic donor was analyzed by flow cytometry. Furthermore, we investigated CD123 and IgG autoantibody expressions. Results: The odds ratio (OR) between ASST and BAT was 1.00 (95% confidence interval [CI]: 0.22 to 4.5). The ASST for autoimmune CU diagnosis showed an accuracy of 54.5%, sensitivity of 66%, specificity of 33%, positive predictive value of 63%, and negative predictive value of 36%. There was no statistical difference between the studied groups as to mean non-specific IgG and CD123 expressions (for a p < 0.05). Discussion: This study demonstrated that ASST has low accuracy in the diagnosis of autoimmune CU. Concerning other analyzed aspects, there was no statistical difference between positive ASST and negative ASST. Conclusions: Due to insufficient studies in this area and the relevance of this issue, further investigation is required.
dc.languagepor
dc.relationJornal Brasileiro de Patologia e Medicina Laboratorial
dc.rightsinfo:eu-repo/semantics/openAccess
dc.subjectAccuracy
dc.subjectAutologous serum skin test
dc.subjectBasophil activation test
dc.subjectCD123
dc.subjectCD63
dc.subjectChronic urticaria
dc.titleCD63 e CD123 expressão, autoanticorpos IgG e acurácia do teste do soro autólogo em pacientes com urticária crônica
dc.typeOtro


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