In vitro effect of low-level laser therapy on typical oral microbial biofilms

dc.contributorUniversidade Estadual Paulista (UNESP)
dc.creatorBasso, Fernanda G.
dc.creatorOliveira, Camila F.
dc.creatorFontana, Amanda
dc.creatorKurachi, Cristina
dc.creatorBagnato, Vanderlei S.
dc.creatorSpolidório, Denise M. P.
dc.creatorHebling, Josimeri
dc.creatorde Souza Costa, Carlos A.
dc.date2014-05-27T11:26:21Z
dc.date2016-10-25T18:36:25Z
dc.date2014-05-27T11:26:21Z
dc.date2016-10-25T18:36:25Z
dc.date2011-12-28
dc.date.accessioned2017-04-06T01:56:42Z
dc.date.available2017-04-06T01:56:42Z
dc.identifierBrazilian Dental Journal, v. 22, n. 6, p. 502-510, 2011.
dc.identifier0103-6440
dc.identifier1806-4760
dc.identifierhttp://hdl.handle.net/11449/73110
dc.identifierhttp://acervodigital.unesp.br/handle/11449/73110
dc.identifier10.1590/S0103-64402011000600011
dc.identifierS0103-64402011000600011
dc.identifier2-s2.0-84255166814.pdf
dc.identifier2-s2.0-84255166814
dc.identifierhttp://dx.doi.org/10.1590/S0103-64402011000600011
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/893935
dc.descriptionThe aim of this study was to evaluate the effect of specific parameters of low-level laser therapy (LLLT) on biofilms formed by Streptococcus mutans, Candida albicans or an association of both species. Single and dual-species biofilms - SSB and DSB - were exposed to laser doses of 5, 10 or 20 J/cm 2 from a near infrared InGaAsP diode laser prototype (LASERTable; 780 ± 3 nm, 0.04 W). After irradiation, the analysis of biobilm viability (MTT assay), biofilm growth (cfu/mL) and cell morphology (SEM) showed that LLLT reduced cell viability as well as the growth of biofilms. The response of S. mutans (SSB) to irradiation was similar for all laser doses and the biofilm growth was dose dependent. However, when associated with C. albicans (DSB), S. mutans was resistant to LLLT. For C. albicans, the association with S. mutans (DSB) caused a significant decrease in biofilm growth in a dose-dependent fashion. The morphology of the microorganisms in the SSB was not altered by LLLT, while the association of microbial species (DSB) promoted a reduction in the formation of C. albicans hyphae. LLLT had an inhibitory effect on the microorganisms, and this capacity can be altered according to the interactions between different microbial species.
dc.languageeng
dc.relationBrazilian Dental Journal
dc.rightsinfo:eu-repo/semantics/openAccess
dc.subjectBiofilm
dc.subjectCandida albicans
dc.subjectLow level laser therapy
dc.subjectStreptococcus mutans
dc.subjectcoloring agent
dc.subjectdiagnostic agent
dc.subjectsuccinate dehydrogenase
dc.subjecttetrazolium
dc.subjectthiazole derivative
dc.subjectthiazolyl blue
dc.subjectbiofilm
dc.subjectcomparative study
dc.subjectdiode laser
dc.subjectfungus hyphae
dc.subjectgrowth, development and aging
dc.subjecthuman
dc.subjectinstrumentation
dc.subjectlow level laser therapy
dc.subjectmaterials testing
dc.subjectmethodology
dc.subjectmicrobial viability
dc.subjectmicrobiological examination
dc.subjectmicrobiology
dc.subjectmouth
dc.subjectmycology
dc.subjectorganismal interaction
dc.subjectradiation dose
dc.subjectradiation exposure
dc.subjectradiation response
dc.subjectscanning electron microscopy
dc.subjecttemperature
dc.subjecttime
dc.subjectultrastructure
dc.subjectBacteriological Techniques
dc.subjectBiofilms
dc.subjectColoring Agents
dc.subjectDose-Response Relationship, Radiation
dc.subjectHumans
dc.subjectHyphae
dc.subjectLaser Therapy, Low-Level
dc.subjectLasers, Semiconductor
dc.subjectMaterials Testing
dc.subjectMicrobial Interactions
dc.subjectMicrobial Viability
dc.subjectMicroscopy, Electron, Scanning
dc.subjectMouth
dc.subjectMycology
dc.subjectRadiation Dosage
dc.subjectSuccinate Dehydrogenase
dc.subjectTemperature
dc.subjectTetrazolium Salts
dc.subjectThiazoles
dc.subjectTime Factors
dc.titleIn vitro effect of low-level laser therapy on typical oral microbial biofilms
dc.typeOtro


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