Salmonella enterica serovar typhimurium colonizing the lumen of the chicken intestine grows slowly and upregulates a unique set of virulence and metabolism genes

dc.contributorUniversidade Estadual Paulista (UNESP)
dc.creatorHarvey, P. C.
dc.creatorWatson, M.
dc.creatorHulme, S.
dc.creatorJones, M. A.
dc.creatorLovell, M.
dc.creatorBerchieri, Jr.
dc.creatorYoung, J.
dc.creatorBumstead, N.
dc.creatorBarrow, P.
dc.date2014-05-27T11:26:01Z
dc.date2016-10-25T18:34:44Z
dc.date2014-05-27T11:26:01Z
dc.date2016-10-25T18:34:44Z
dc.date2011-10-01
dc.date.accessioned2017-04-06T01:52:40Z
dc.date.available2017-04-06T01:52:40Z
dc.identifierInfection and Immunity, v. 79, n. 10, p. 4105-4121, 2011.
dc.identifier0019-9567
dc.identifier1098-5522
dc.identifierhttp://hdl.handle.net/11449/72708
dc.identifierhttp://acervodigital.unesp.br/handle/11449/72708
dc.identifier10.1128/IAI.01390-10
dc.identifier2-s2.0-80855136572.pdf
dc.identifier2-s2.0-80855136572
dc.identifierhttp://dx.doi.org/10.1128/IAI.01390-10
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/893554
dc.descriptionThe pattern of global gene expression in Salmonella enterica serovar Typhimurium bacteria harvested from the chicken intestinal lumen (cecum) was compared with that of a late-log-phase LB broth culture using a whole-genome microarray. Levels of transcription, translation, and cell division in vivo were lower than those in vitro. S. Typhimurium appeared to be using carbon sources, such as propionate, 1,2-propanediol, and ethanolamine, in addition to melibiose and ascorbate, the latter possibly transformed to D-xylulose. Amino acid starvation appeared to be a factor during colonization. Bacteria in the lumen were non- or weakly motile and nonchemotactic but showed upregulation of a number of fimbrial and Salmonella pathogenicity island 3 (SPI-3) and 5 genes, suggesting a close physical association with the host during colonization. S. Typhimurium bacteria harvested from the cecal mucosa showed an expression profile similar to that of bacteria from the intestinal lumen, except that levels of transcription, translation, and cell division were higher and glucose may also have been used as a carbon source. © 2011, American Society for Microbiology.
dc.languageeng
dc.relationInfection and Immunity
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.subjectarginine
dc.subjectascorbic acid
dc.subjectcarbon
dc.subjectcitrate synthase
dc.subjectDNA binding protein
dc.subjectDNA directed DNA polymerase gamma
dc.subjectethanolamine
dc.subjectflagellin
dc.subjectglucose
dc.subjecthost factor 1
dc.subjectmelibiose
dc.subjectpropionic acid
dc.subjectpropylene glycol
dc.subjectputrescine derivative
dc.subjectthreonine dehydratase
dc.subjectxylulose
dc.subjectamino acid metabolism
dc.subjectamino acid transport
dc.subjectanimal tissue
dc.subjectbacterial colonization
dc.subjectcarbon source
dc.subjectcecum
dc.subjectcell division
dc.subjectchromosome replication
dc.subjectcontrolled study
dc.subjectcross reaction
dc.subjectDNA replication
dc.subjectdown regulation
dc.subjectenergy yield
dc.subjectflagellum
dc.subjectgene control
dc.subjectgene expression
dc.subjectgenetic transcription
dc.subjectin vitro study
dc.subjectin vivo study
dc.subjectintestine
dc.subjectintestine mucosa
dc.subjectnonhuman
dc.subjectpathogenicity
dc.subjectpriority journal
dc.subjectSalmonella enterica
dc.subjectSalmonella typhimurium
dc.subjectupregulation
dc.subjectvirulence
dc.subjectAnimals
dc.subjectBacterial Proteins
dc.subjectCecum
dc.subjectChickens
dc.subjectGene Expression Profiling
dc.subjectGene Expression Regulation, Bacterial
dc.subjectGenome, Bacterial
dc.subjectMice
dc.subjectMice, Inbred BALB C
dc.subjectOligonucleotide Array Sequence Analysis
dc.subjectPoultry Diseases
dc.subjectSalmonella Infections, Animal
dc.subjectSpecific Pathogen-Free Organisms
dc.subjectUp-Regulation
dc.subjectVirulence
dc.subjectVirulence Factors
dc.titleSalmonella enterica serovar typhimurium colonizing the lumen of the chicken intestine grows slowly and upregulates a unique set of virulence and metabolism genes
dc.typeOtro


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