Otro
Agaricus blazei as a Substrate for the Production of β-1,3-Glucanase by Trichoderma harzianum Rifai
Registro en:
Food Technology and Biotechnology, v. 49, n. 3, p. 341-346, 2011.
1330-9862
1334-2606
2-s2.0-80053337073.pdf
2-s2.0-80053337073
Autor
Carneiro, Andréia A.J.
Giese, Ellen C.
Barbosa, Aneli M.
Gomes, Eleni
Da Silva, Roberto
Resumen
Extracellular β-1,3-glucanase was produced by Trichoderma harzianum Rifai cultivated in the Agaricus blazei (Agaricus brasiliensis) extract as a substrate in submerged fermentation. A 22-central composite factorial design was developed using the time of culture (x1/day) and Agaricus blazei extract concentration (x2/(g/L)) as variables, and the results were analyzed using response surface methodology (RSM). The results showed that the Agaricus blazei extract concentration was the most important variable in the production of β-1,3-glucanase, and the maximum β-1,3-glucanase activity (0.77 U/mL) was obtained in one day of cultivation. The β-glucan present in the cell wall of Agaricus blazei mushroom proved to be a good substrate for inducing the production of specific β-1,3-glucanase by Trichoderma harzianum Rifai.