Evolutionary dynamics of 5S rDNA location in acridid grasshoppers and its relationship with H3 histone gene and 45S rDNA location

dc.contributorUniversidade Estadual Paulista (UNESP)
dc.creatorCabral-de-Mello, Diogo C.
dc.creatorCabrero, Josefa
dc.creatorLópez-León, María Dolores
dc.creatorCamacho, Juan Pedro M.
dc.date2014-05-27T11:25:55Z
dc.date2016-10-25T18:34:06Z
dc.date2014-05-27T11:25:55Z
dc.date2016-10-25T18:34:06Z
dc.date2011-07-01
dc.date.accessioned2017-04-06T01:51:04Z
dc.date.available2017-04-06T01:51:04Z
dc.identifierGenetica, v. 139, n. 7, p. 921-931, 2011.
dc.identifier0016-6707
dc.identifierhttp://hdl.handle.net/11449/72507
dc.identifierhttp://acervodigital.unesp.br/handle/11449/72507
dc.identifier10.1007/s10709-011-9596-7
dc.identifier2-s2.0-79960845609
dc.identifierhttp://dx.doi.org/10.1007/s10709-011-9596-7
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/893372
dc.descriptionWe analyze the chromosomal location of 5S rDNA clusters in 29 species of grasshoppers belonging to the family Acrididae. There was extensive variation among species for the number and location of 5S rDNA sites. Out of 148 sites detected, 75% were proximally located, 21.6% were interstitial, and only 3.4% were distal. The number of 5S rDNA sites per species varied from a single chromosome pair (in six species) to all chromosome pairs (in five species), with a range of intermediate situations. Thirteen chromosomes from eight species carried two 5S rDNA clusters. At intraspecific level, differences among populations were detected in Eyprepocnemis plorans, and some heteromorphisms have also been observed in some species. Double FISH for 5S rDNA and H3 histone gene DNA, performed on 17 of these 29 species, revealed that both markers are sometimes placed in a same chromosome but at different location, whereas they appeared to co-localize in five species (Calliptamus barbarus, Heteracris adpersa, Aiolopus strepens, Oedipoda charpentieri and O. coerulescens). Double fiber-FISH in A. strepens and O. coerulescens showed that the two DNAs are closely interspersed with variable relative amounts of both classes of DNA. Finally, no correlation was observed between the number of 5S and 45S rDNA clusters in 23 species where this information was available. These results are discussed in the light of possible mechanisms of spread that led to the extensive variation in the number of clusters observed for both rDNA types in acridid grasshoppers. © 2011 Springer Science+Business Media B.V.
dc.languageeng
dc.relationGenetica
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.subjectFluorescence in situ hybridization
dc.subjectGrasshopper
dc.subjectHistone genes
dc.subjectMultigene families
dc.subjectrDNA
dc.subjecthistone
dc.subjectribosome DNA
dc.subjectribosome RNA
dc.subjectRNA 5S
dc.subjectRNA, ribosomal, 45S
dc.subjectanimal
dc.subjectCaelifera
dc.subjectchromosome map
dc.subjectcytology
dc.subjectevolution
dc.subjectfluorescence in situ hybridization
dc.subjectgenetics
dc.subjectGreece
dc.subjectkaryotyping
dc.subjectmale
dc.subjectmethodology
dc.subjectMorocco
dc.subjectmultigene family
dc.subjectSpain
dc.subjectspecies difference
dc.subjectAnimals
dc.subjectBiological Evolution
dc.subjectChromosome Mapping
dc.subjectDNA, Ribosomal
dc.subjectGrasshoppers
dc.subjectHistones
dc.subjectIn Situ Hybridization, Fluorescence
dc.subjectKaryotyping
dc.subjectMale
dc.subjectMultigene Family
dc.subjectRNA, Ribosomal
dc.subjectRNA, Ribosomal, 5S
dc.subjectSpecies Specificity
dc.subjectAcrididae
dc.subjectAiolopus
dc.subjectCalliptamus barbarus
dc.subjectEyprepocnemis plorans
dc.subjectOedipoda
dc.titleEvolutionary dynamics of 5S rDNA location in acridid grasshoppers and its relationship with H3 histone gene and 45S rDNA location
dc.typeOtro


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