Mast cells modulate the inflammatory process in endotoxin-induced uveitis

dc.contributorUniversidade Estadual Paulista (UNESP)
dc.creatorSilva, Pierre Sebastião da
dc.creatorGiro, Ana Paula
dc.creatorOliani, Sonia M.
dc.date2014-05-27T11:25:53Z
dc.date2016-10-25T18:33:56Z
dc.date2014-05-27T11:25:53Z
dc.date2016-10-25T18:33:56Z
dc.date2011-05-30
dc.date.accessioned2017-04-06T01:50:33Z
dc.date.available2017-04-06T01:50:33Z
dc.identifierMolecular Vision, v. 17, p. 1310-1319.
dc.identifier1090-0535
dc.identifierhttp://hdl.handle.net/11449/72440
dc.identifierhttp://acervodigital.unesp.br/handle/11449/72440
dc.identifier2-s2.0-79957510239.pdf
dc.identifier2-s2.0-79957510239
dc.identifierhttp://www.ncbi.nlm.nih.gov/pmc/articles/PMC3103740/
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/893308
dc.descriptionPurpose: To investigate the role of mast cells and annexin-A1 (Anxa1) in endotoxin-induced uveitis (EIU). Methods: EIU was induced by injection of lipopolysaccharide (LPS) into the paws of rats, which were then sacrificed after 24 and 48 h. To assess EIU in the absence of mast cells, groups of animals were pretreated with compound 48/80 (c48/80) and sacrificed after 24 h after no treatment or EIU induction. The eyes were used for histological studies and the aqueous humor (AqH) pool was used for the analysis of transmigrated cells and Anxa1 levels. In inflammatory cells, Anxa1 expression was monitored by immunohistochemistry. Results: After 24 h, rats with EIU exhibited degranulated mast cells, associated with elevated numbers of infiltrating leukocytes and the high expression of Anxa1 in the AqH and the neutrophils. After 48 h of EIU, the mast cells were intact, indicating granule re-synthesis, and there was a reduction of neutrophil transmigration and an increase in the number of mononuclear phagocytic cells in ocular tissues. Anxa1 expression was decreased in neutrophils but increased in mononuclear phagocytic cells. In the animals pretreated with c48/80 and subjected to EIU, mast cells responded to this secretagogue by degranulating and few transmigrated neutrophils were observed. Conclustions: We report that mast cells are a potential source of pharmacological mediators that are strongly linked to the pathophysiology of EIU, and the endogenous protein Anxa1 is a mediator in the homeostasis of the inflammatory process with anti-migratory effects on leukocytes, which supports further studies of this protein as an innovative therapy for uveitis. © 2011 Molecular Vision.
dc.languageeng
dc.relationMolecular Vision
dc.rightsinfo:eu-repo/semantics/openAccess
dc.subjectlipocortin 1
dc.subjectanimal cell
dc.subjectanimal experiment
dc.subjectanimal model
dc.subjectanimal tissue
dc.subjectaqueous humor
dc.subjectcell granule
dc.subjectcell infiltration
dc.subjectcell migration
dc.subjectcontrolled study
dc.subjectdisease course
dc.subjectinflammatory cell
dc.subjectleukocyte count
dc.subjectmast cell
dc.subjectmast cell degranulation
dc.subjectmononuclear cell
dc.subjectneutrophil chemotaxis
dc.subjectnonhuman
dc.subjectpathophysiology
dc.subjectphagocyte
dc.subjectpriority journal
dc.subjectprotein expression
dc.subjectprotein function
dc.subjectprotein synthesis
dc.subjectrat
dc.subjectuveitis
dc.subjectAnimals
dc.subjectAnnexin A1
dc.subjectAqueous Humor
dc.subjectBlotting, Western
dc.subjectCell Count
dc.subjectCell Degranulation
dc.subjectCell Movement
dc.subjectEye
dc.subjectEye Proteins
dc.subjectHomeostasis
dc.subjectImmunohistochemistry
dc.subjectLeukocytes
dc.subjectLipopolysaccharides
dc.subjectMale
dc.subjectMast Cells
dc.subjectNeutrophils
dc.subjectp-Methoxy-N-methylphenethylamine
dc.subjectRats
dc.subjectRats, Wistar
dc.subjectUveitis
dc.titleMast cells modulate the inflammatory process in endotoxin-induced uveitis
dc.typeOtro


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