Formação de biofilme cristalino de Proteus mirabilis em cateter urológico

dc.contributorUniversidade Estadual Paulista (UNESP)
dc.creatordos Santos, Cássio Antonio Lanfredi
dc.creatorde Campos Júnior, Flávio Ferraz
dc.creatorPizzolitto, Antonio Carlos
dc.creatorPizzolitto, Elisabeth Loshchagin
dc.date2014-05-27T11:25:25Z
dc.date2016-10-25T18:33:17Z
dc.date2014-05-27T11:25:25Z
dc.date2016-10-25T18:33:17Z
dc.date2010-12-03
dc.date.accessioned2017-04-06T01:48:37Z
dc.date.available2017-04-06T01:48:37Z
dc.identifierRevista Brasileira de Engenharia Biomedica, v. 26, n. 2, p. 91-98, 2010.
dc.identifier1517-3151
dc.identifier1984-7742
dc.identifierhttp://hdl.handle.net/11449/72207
dc.identifierhttp://acervodigital.unesp.br/handle/11449/72207
dc.identifier2-s2.0-78651531815.pdf
dc.identifier2-s2.0-78651531815
dc.identifierhttp://www.rbeb.org.br/articles/view/id/4dfa530418626adbef000008
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/893100
dc.descriptionThe indwelling urethral catheter has an important role for patients with urinary retention, bladder obstruction, neurological damage and other diseases. Urine contains minerals which precipitate in alkaline pH, crystallize and block the urological catheter. The crystallization of the ionic components of urine occurs in the presence of urease, an enzyme produced by Proteus mirabilis. This bacterium adheres to inanimate surfaces and forms biofilms. The aim of this study was to investigate the formation of crystalline biofilm on the luminal surface of siliconized latex catheters by means of scanning electron microscope, after channeling artificial urine infected with Proteus mirabilis. The experiment was performed in vitro using a dynamic flow system. The artificial urine compounds were salts of calcium, magnesium, phosphates, urea and egg albumin, and it was infected with Proteus mirabilis ATCC 25933. The urine flow was stopped after crystallization of the ionic components. Crystallization was observed after alkalinization of urine. Scanning electron microscopy showed the presence of crystals and morphologies typical of bacilli embedded in an amorphous mass on the internal lumen of the catheter. The present study showed that catheter encrustation may limit the use of long-term indwelling catheter. © SBEB - Sociedade Brasileira de Engenharia Biomédica.
dc.languageeng
dc.languagepor
dc.relationRevista Brasileira de Engenharia Biomédica
dc.rightsinfo:eu-repo/semantics/openAccess
dc.subjectArtificial urine
dc.subjectCrystalline biofilm
dc.subjectEncrustation of catheters
dc.subjectFoley catheter
dc.subjectProteus mirabilis
dc.subjectBiofilms
dc.subjectBiofilters
dc.subjectBody fluids
dc.subjectCrystalline materials
dc.subjectCrystallization
dc.subjectMagnesium
dc.subjectScanning electron microscopy
dc.subjectUrea
dc.subjectCatheters
dc.titleFormação de biofilme cristalino de Proteus mirabilis em cateter urológico
dc.typeOtro


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