dc.contributorUniversidade Estadual Paulista (UNESP)
dc.creatorda Costa, Anna Carolina Borges Pereira
dc.creatorChibebe Junior, José
dc.creatorPereira, Cristiane Aparecida
dc.creatorMachado, Ana Karina da Silva
dc.creatorBeltrame Junior, Milton
dc.creatorJunqueira, Juliana Campos
dc.creatorJorge, Antonio Olavo Cardoso
dc.date2014-05-27T11:24:48Z
dc.date2016-10-25T18:29:08Z
dc.date2014-05-27T11:24:48Z
dc.date2016-10-25T18:29:08Z
dc.date2010-10-01
dc.date.accessioned2017-04-06T01:43:07Z
dc.date.available2017-04-06T01:43:07Z
dc.identifierBrazilian Oral Research, v. 24, n. 4, p. 413-418, 2010.
dc.identifier1806-8324
dc.identifier1807-3107
dc.identifierhttp://hdl.handle.net/11449/71906
dc.identifierhttp://acervodigital.unesp.br/handle/11449/71906
dc.identifier10.1590/S1806-83242010000400007
dc.identifierS1806-83242010000400007
dc.identifier2-s2.0-78650628907.pdf
dc.identifier2-s2.0-78650628907
dc.identifierhttp://dx.doi.org/10.1590/S1806-83242010000400007
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/892823
dc.descriptionThe objective of this study was to evaluate the effect of photodynamic therapy with erythrosine and rose bengal using a light-emitting diode (LED) on planktonic cultures of S. mutans. Ten S. mutans strains, including nine clinical strains and one reference strain (ATCC 35688), were used. Suspensions containing 10 6 cells/mL were prepared for each strain and were tested under different experimental conditions: a) LED irradiation in the presence of rose bengal as a photosensitizer (RB+L+); b) LED irradiation in the presence of erythrosine as a photosensitizer (E+L+); c) LED irradiation only (P-L+); d) treatment with rose bengal only (RB+L-); e) treatment with erythrosine only (E+L-); and f) no LED irradiation or photosensitizer treatment, which served as a control group (P-L-). After treatment, the strains were seeded onto BHI agar for determination of the number of colony-forming units (CFU/mL). The results were submitted to analysis of variance and the Tukey test (p ≤ 0.05). The number of CFU/mL was significantly lower in the groups submitted to photodynamic therapy (RB+L+ and E+L+) compared to control (P-L-), with a reduction of 6.86 log 10 in the RB+L+ group and of 5.16 log 10 in the E+L+ group. Photodynamic therapy with rose bengal and erythrosine exerted an antimicrobial effect on all S. mutans strains studied.
dc.languageeng
dc.relationBrazilian Oral Research
dc.rightsinfo:eu-repo/semantics/openAccess
dc.subjectErythrosine
dc.subjectPhotochemotherapy
dc.subjectRose bengal
dc.subjectStreptococcus mutans
dc.subjecterythrosine
dc.subjectphotosensitizing agent
dc.subjectrose bengal
dc.subjectanalysis of variance
dc.subjectbacterial load
dc.subjectbiofilm
dc.subjectcell culture
dc.subjectdental caries
dc.subjectdrug effect
dc.subjectisolation and purification
dc.subjectmethodology
dc.subjectphotochemotherapy
dc.subjectradiation exposure
dc.subjecttime
dc.subjectAnalysis of Variance
dc.subjectBacterial Load
dc.subjectBiofilms
dc.subjectCells, Cultured
dc.subjectDental Caries
dc.subjectPhotosensitizing Agents
dc.subjectRose Bengal
dc.subjectTime Factors
dc.titleSusceptibility of planktonic cultures of Streptococcus mutans to photodynamic therapy with a light-emitting diode
dc.typeOtro


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