The Rv1712 locus from Mycobacterium tuberculosis H37Rv codes for a functional CMP kinase that preferentially Phosphorylates dCMP

Thum, Caroline; Schneider, Cristopher Z.; Palma, Mario Sergio; Santos, Diógenes S.; Basso, Luiz A.

Otro

Registro en:

Journal of Bacteriology, v. 191, n. 8, p. 2884-2887, 2009.

0021-9193

http://hdl.handle.net/11449/70966

http://acervodigital.unesp.br/handle/11449/70966

10.1128/JB.01337-08

2-s2.0-65249143233.pdf

2-s2.0-65249143233

http://dx.doi.org/10.1128/JB.01337-08

http://repositorioslatinoamericanos.uchile.cl/handle/2250/892020

Autor

Thum, Caroline

Schneider, Cristopher Z.

Palma, Mario Sergio

Santos, Diógenes S.

Basso, Luiz A.

Institución

Universidade Paulista Júlio de Mesquita Filho (Brasil)

Resumen

The Mycobacterium tuberculosis cmk gene, predicted to encode a CMP kinase (CMK), was cloned and expressed, and its product was purified to homogeneity. Steady-state kinetics confirmed that M. tuberculosis CMK is a monomer that preferentially phosphorylates CMP and dCMP by a sequential mechanism. A plausible role for CMK is discussed. Copyright © 2009, American Society for Microbiology. All Rights Reserved.