What's the signification of nuclear vacuoles in human sperm?

dc.contributorUniversidade Estadual Paulista (UNESP)
dc.creatorFranco, J. G.
dc.creatorBaruffi, R. L R
dc.creatorMauri, A. L.
dc.creatorPetersen, C. G.
dc.creatorOliveira, J. B A
dc.creatorVagnini, L.
dc.date2014-05-27T11:23:35Z
dc.date2016-10-25T18:25:42Z
dc.date2014-05-27T11:23:35Z
dc.date2016-10-25T18:25:42Z
dc.date2008-07-01
dc.date.accessioned2017-04-06T01:31:26Z
dc.date.available2017-04-06T01:31:26Z
dc.identifierJornal Brasileiro de Reproducao Assistida, v. 12, n. 3, p. 16-19, 2008.
dc.identifier1517-5693
dc.identifier1518-0557
dc.identifierhttp://hdl.handle.net/11449/70471
dc.identifierhttp://acervodigital.unesp.br/handle/11449/70471
dc.identifier2-s2.0-67649610295
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/891568
dc.descriptionThe aim of this study was to determine the extent of DNA fragmentation and the presence of single/denatured or double stranded of DNA in sperm with large nuclear vacuoles (LNV) selected by high-magnification. A total of 30 patients had fresh semen samples prepared by discontinuous concentration gradient. Sperm with normal nucleus (NN) and LNV were selected at 8400x magnification and placed in different slides. DNA fragmentation was determined by TUNEL assay. Denatured and double stranded DNA was identified by acridine orange fluorescence method. The percentage of DNA fragmentation in LNV sperm (29%) was significantly higher (P<0.001) than NN sperm (15.8%). Therefore, cleavage of genomic DNA in low molecular weight DNA fragments (mono and oligonucleosomes), and single strand breaks (nicks) in high molecular weight DNA occur more frequently in LNV. Identically, the percentage denatured stranded DNA in sperm with LNV (67.9%) was significantly higher (P <0.0001) than NN sperm (33%). The high level of denatured DNA in sperm with LNV suggests precocious decondensation and disaggregation of sperm chromatin fibers. Our results support an association between LNV sperm and DNA damage, and the routine selection and injection of morphological motile sperm at high magnification for ICSI. The adverse effect (DNA fragmentation or denaturation) leads to concern particularly about the possibility of iatrogenic transmission of genetic abnormalities. Copyright - SBRA - Sociedade Brasileira de Reprodução Assistida.
dc.languageeng
dc.relationJornal Brasileiro de Reproducao Assistida
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.subjectDenatured stranded DNA
dc.subjectDNA fragmentation
dc.subjectHuman sperm
dc.subjectICSI
dc.subjectNuclear vacuoles
dc.subjectacridine orange
dc.subjectdouble stranded DNA
dc.subjectgenomic DNA
dc.subjectsingle stranded DNA
dc.subjectcell nucleus
dc.subjectcell vacuole
dc.subjectDNA cleavage
dc.subjectDNA damage
dc.subjectDNA denaturation
dc.subjectfluorescence
dc.subjectgenetic disorder
dc.subjecthuman
dc.subjectiatrogenic disease
dc.subjectmale
dc.subjectnick end labeling
dc.subjectsperm
dc.titleWhat's the signification of nuclear vacuoles in human sperm?
dc.typeOtro


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