Optimal clone identifier for genomic shotgun libraries: OC Identifier tool

dc.contributorUniversidade Estadual Paulista (UNESP)
dc.creatorCantão, M. E.
dc.creatorFerreira, J. E.
dc.creatorLemos, E. G. M.
dc.date2014-05-27T11:22:45Z
dc.date2016-10-25T18:25:04Z
dc.date2014-05-27T11:22:45Z
dc.date2016-10-25T18:25:04Z
dc.date2007-12-28
dc.date.accessioned2017-04-06T01:29:22Z
dc.date.available2017-04-06T01:29:22Z
dc.identifierGenetics and Molecular Research, v. 6, n. 4, p. 743-755, 2007.
dc.identifier1676-5680
dc.identifierhttp://hdl.handle.net/11449/70219
dc.identifierhttp://acervodigital.unesp.br/handle/11449/70219
dc.identifierWOS:000251697000003
dc.identifier2-s2.0-37349108551.pdf
dc.identifier2-s2.0-37349108551
dc.identifierhttp://www.geneticsmr.com//year2007/vol6-4/pdf/xm0002.pdf
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/891360
dc.descriptionIn DNA microarray experiments, the gene fragments that are spotted on the slides are usually obtained by the synthesis of specific oligonucleotides that are able to amplify genes through PCR. Shotgun library sequences are an alternative to synthesis of primers for the study of each gene in the genome. The possibility of putting thousands of gene sequences into a single slide allows the use of shotgun clones in order to proceed with microarray analysis without a completely sequenced genome. We developed an OC Identifier tool (optimal clone identifier for genomic shotgun libraries) for the identification of unique genes in shotgun libraries based on a partially sequenced genome; this allows simultaneous use of clones in projects such as transcriptome and phylogeny studies, using comparative genomic hybridization and genome assembly. The OC Identifier tool allows comparative genome analysis, biological databases, query language in relational databases, and provides bioinformatics tools to identify clones that contain unique genes as alternatives to primer synthesis. The OC Identifier allows analysis of clones during the sequencing phase, making it possible to select genes of interest for construction of a DNA microarray. ©FUNPEC-RP.
dc.languageeng
dc.relationGenetics and Molecular Research
dc.rightsinfo:eu-repo/semantics/openAccess
dc.subjectBioinformatics tool
dc.subjectComparative genomic hybridization
dc.subjectDNA microarray
dc.subjectGene expression
dc.subjectGenomic shotgun libraries
dc.subjectOptimal clones
dc.subjectDNA fragment
dc.subjectoligonucleotide
dc.subjecttranscriptome
dc.subjectbioinformatics
dc.subjectconference paper
dc.subjectfactual database
dc.subjectgene sequence
dc.subjectgenetic algorithm
dc.subjectgenome
dc.subjectgenome analysis
dc.subjectmolecular cloning
dc.subjectmolecular library
dc.subjectmolecular phylogeny
dc.subjectnucleotide metabolism
dc.subjectopen reading frame
dc.subjectpolymerase chain reaction
dc.subjectsequence analysis
dc.subjectClone Cells
dc.subjectCloning, Molecular
dc.subjectComputational Biology
dc.subjectGenome, Bacterial
dc.subjectGenomic Library
dc.subjectOpen Reading Frames
dc.subjectSoftware
dc.titleOptimal clone identifier for genomic shotgun libraries: OC Identifier tool
dc.typeOtro


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