Immunohistochemistry assay to detect Turkey coronavirus (TCoV) from experimentally infected poults

dc.contributorUniversidade Estadual Paulista (UNESP)
dc.creatorCastanheira, Thais Larissa L.
dc.creatorFerrari, Heitor F.
dc.creatorLuvizotto, Maria Cecília R.
dc.creatorCardoso, Tereza C.
dc.date2014-05-27T11:22:42Z
dc.date2016-10-25T18:24:47Z
dc.date2014-05-27T11:22:42Z
dc.date2016-10-25T18:24:47Z
dc.date2007-12-01
dc.date.accessioned2017-04-06T01:28:19Z
dc.date.available2017-04-06T01:28:19Z
dc.identifierAmerican Journal of Immunology, v. 3, n. 2, p. 31-34, 2007.
dc.identifier1553-619X
dc.identifierhttp://hdl.handle.net/11449/70097
dc.identifierhttp://acervodigital.unesp.br/handle/11449/70097
dc.identifier10.3844/ajisp.2007.31.34
dc.identifier2-s2.0-38749109226
dc.identifierhttp://dx.doi.org/10.3844/ajisp.2007.31.34
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/891245
dc.descriptionThe objective of this study was to develop a direct immunohistochemical assay to detect TCoV antigens in formalin-fixed paraffin-embedded sections prepared from experimentally infected poults. The sections of ileo, ileo-cecal junction and ceca regions from intestine were prepared and submitted to two different primary antibodies, first the non-biotin labeled polyclonal antibody for the indirect method, and second the biotin-labeled polyclonal antibody, both raised against IBV by immunized specific pathogen free chickens. All sections were submitted to immufluorescent assay (IFA), a conventional method, and the results compared. The direct immunohistochemical technique showed a higher frequency of antigen in tissues, especially from the ileo-cecal junction with no difference between results obtained by the conventional method. Finally, the immunofluorescence and all modalities of molecular approaches have been played an important role to the diagnosis and prevention of TCoV infections, although to be precise on infectious disease diagnosis, it is necessary complementary techniques. Here, was standardized the biotin labeled polyclonal antibody as reliable tool to be used as an alternative detection of Turkey Coronavirus. © 2007 Science Publications.
dc.descriptionFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.languageeng
dc.relationAmerican Journal of Immunology
dc.rightsinfo:eu-repo/semantics/openAccess
dc.subjectIFA
dc.subjectImmunohistochemistry
dc.subjectPEMS
dc.subjectTCoV
dc.subjectbiotin
dc.subjectpolyclonal antibody
dc.subjectanimal experiment
dc.subjectanimal model
dc.subjectanimal tissue
dc.subjectantibody labeling
dc.subjectantigen detection
dc.subjectAvian infectious bronchitis virus
dc.subjectbird disease
dc.subjectcontrolled study
dc.subjectCoronavirus
dc.subjectembryo
dc.subjecthistopathology
dc.subjectimmunization
dc.subjectimmunofluorescence
dc.subjectimmunohistochemistry
dc.subjectintermethod comparison
dc.subjectintestine infection
dc.subjectnonhuman
dc.subjectturkey (bird)
dc.subjectvirus detection
dc.subjectvirus infection
dc.titleImmunohistochemistry assay to detect Turkey coronavirus (TCoV) from experimentally infected poults
dc.typeOtro


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