Immunization of Balb/c mice with modified auto-antigens for induction of autoimmune sialoadenitis

dc.contributorUniversidade Estadual Paulista (UNESP)
dc.creatorUnterkircher, Carmelinda Schmidt
dc.creatorLeão, Mariella Vieira Pereira
dc.creatorCarvalho, Yasmin Rodarte
dc.date2014-05-27T11:22:41Z
dc.date2016-10-25T18:24:42Z
dc.date2014-05-27T11:22:41Z
dc.date2016-10-25T18:24:42Z
dc.date2007-12-01
dc.date.accessioned2017-04-06T01:28:02Z
dc.date.available2017-04-06T01:28:02Z
dc.identifierBrazilian Dental Journal, v. 18, n. 1, p. 40-44, 2007.
dc.identifier0103-6440
dc.identifier1806-4760
dc.identifierhttp://hdl.handle.net/11449/70065
dc.identifierhttp://acervodigital.unesp.br/handle/11449/70065
dc.identifier10.1590/S0103-64402007000100009
dc.identifierS0103-64402007000100009
dc.identifier2-s2.0-34848928990.pdf
dc.identifier2-s2.0-34848928990
dc.identifierhttp://dx.doi.org/10.1590/S0103-64402007000100009
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/891216
dc.descriptionSjögren's syndrome is an autoimmune disease characterized by sialoadenitis and elevated titers of autoantibodies. To assess whether it is possible to induce inflammatory changes in salivary gland tissues, a series of immunizations in Balb/c mice have been undertaken, using salivary gland extract, modified or not, added to several adjuvants. Mice's humoral immune response to salivary gland antigens was monitored by ELISA. Inflammatory cells infiltrating gland tissue were seen 3 months after immunization with salivary gland extract modified with pepsin (AgGp) and metaperiodate (AgGMp). Although pathological progression was not observed, the histopathological picture was similar to the initial phase of Sjögren's syndrome. In addition, a monoclonal antibody reactive with 3 gland polypeptides and anhydrase carbonic II was rescued among B cells from immunized mice. Thus, immunizations with modified autoantigens were able to initiate pathological damage to glandular tissue and stimulate the proliferation of auto-reactive B cells.
dc.languageeng
dc.relationBrazilian Dental Journal
dc.rightsinfo:eu-repo/semantics/openAccess
dc.subjectAutoimmune disease
dc.subjectCarbonic anhydrase
dc.subjectSalivary gland
dc.subjectSialoadenitis
dc.subjectautoantigen
dc.subjectmitogenic agent
dc.subjectperiodate
dc.subjectperiodic acid
dc.subjectanimal
dc.subjectBagg albino mouse
dc.subjectcattle
dc.subjectfemale
dc.subjecthybridoma
dc.subjectimmunology
dc.subjectmouse
dc.subjectpathology
dc.subjectsalivary gland
dc.subjectsialoadenitis
dc.subjectSjoegren syndrome
dc.subjectvaccination
dc.subjectAnimals
dc.subjectAutoantigens
dc.subjectCattle
dc.subjectFemale
dc.subjectHybridomas
dc.subjectMice
dc.subjectMice, Inbred BALB C
dc.subjectMitogens
dc.subjectPeriodic Acid
dc.subjectSalivary Glands
dc.subjectSialadenitis
dc.subjectSjogren's Syndrome
dc.subjectVaccination
dc.titleImmunization of Balb/c mice with modified auto-antigens for induction of autoimmune sialoadenitis
dc.typeOtro


Este ítem pertenece a la siguiente institución