HPLC determination of hemoglobins to establish reference values with the aid of statistics and informatics

dc.contributorUniversidade Estadual Paulista (UNESP)
dc.creatorOndei, L. S.
dc.creatorZamaro, P. J A
dc.creatorMangonaro, P. H.
dc.creatorValêncio, C. R.
dc.creatorBonini-Domingos, C. R.
dc.date2014-05-27T11:22:35Z
dc.date2016-10-25T18:24:18Z
dc.date2014-05-27T11:22:35Z
dc.date2016-10-25T18:24:18Z
dc.date2007-09-03
dc.date.accessioned2017-04-06T01:26:29Z
dc.date.available2017-04-06T01:26:29Z
dc.identifierGenetics and Molecular Research, v. 6, n. 2, p. 453-460, 2007.
dc.identifier1676-5680
dc.identifierhttp://hdl.handle.net/11449/69878
dc.identifierhttp://acervodigital.unesp.br/handle/11449/69878
dc.identifierWOS:000251696400023
dc.identifier2-s2.0-34548293860.pdf
dc.identifier2-s2.0-34548293860
dc.identifierhttp://www.geneticsmr.com//year2007/vol6-2/pdf/gmr0312.pdf
dc.identifierhttp://www.geneticsmr.com/articles/371
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/891055
dc.descriptionThe purpose of the present study was to establish reference values for hemoglobins (Hb) using HPLC, in samples containing normal Hb (AA), sickle cell trait without alpha-thalassemia (AS), sickle cell trait with alpha-thalassemia (ASH), sickle cell anemia (SS), and Hb SC disease (SC). The blood samples were analyzed by electrophoresis, HPLC and molecular procedures. The Hb A2 mean was 4.30 ± 0.44% in AS, 4.18 ± 0.42% in ASH, 3.90 ± 1.14% in SS, and 4.39 ± 0.35% in SC. They were similar, but above the normal range. Between the AS and ASH groups, only the amount of Hb S was higher in the AS group. The Hb S mean in the AS group was 38.54 ± 3.01% and in the ASH it was 36.54 ± 3.76%. In the qualitative analysis, using FastMap, distinct groups were seen: AA and SS located at opposite extremes, AS and ASH with overlapping values and intermediate distribution, SC between heterozygotes and the SS group. Hb S was confirmed by allele-specific polymerase chain reaction. The Hb values established will be available for use as a reference for the Brazilian population, drawing attention to the increased levels of Hb A2, which should be considered with caution to prevent incorrect diagnoses. ©FUNPEC-RP.
dc.languageeng
dc.relationGenetics and Molecular Research
dc.rightsinfo:eu-repo/semantics/openAccess
dc.subjectHemoglobins
dc.subjectHigh-performance liquid chromatography
dc.subjectReference values
dc.subjecthemoglobin
dc.subjecthemoglobin A
dc.subjecthemoglobin A2
dc.subjecthemoglobin C
dc.subjecthemoglobin F
dc.subjecthemoglobin S
dc.subjectalpha thalassemia
dc.subjectblood sampling
dc.subjectcontrolled study
dc.subjectelectrophoresis
dc.subjecthemoglobin determination
dc.subjecthigh performance liquid chromatography
dc.subjecthuman
dc.subjectphenotype
dc.subjectreference value
dc.subjectsickle cell anemia
dc.subjectsickle cell trait
dc.subjectalpha-Thalassemia
dc.subjectBrazil
dc.subjectChromatography, High Pressure Liquid
dc.subjectComputational Biology
dc.subjectElectrophoresis
dc.subjectGenetic Techniques
dc.subjectHeterozygote
dc.subjectHumans
dc.subjectModels, Statistical
dc.subjectMutation
dc.subjectPhenotype
dc.subjectReference Values
dc.subjectSickle Cell Trait
dc.titleHPLC determination of hemoglobins to establish reference values with the aid of statistics and informatics
dc.typeOtro


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