The effect of CpG-ODN on antigen presenting cells of the foal

dc.contributorUniversidade Estadual Paulista (UNESP)
dc.creatorJulia, M. Julia B.F.
dc.creatorBorges, Alexandre Secorun
dc.creatorNydam, Daryl V.
dc.creatorHorohov, David W.
dc.creatorHecker, Rolf
dc.creatorMatychak, Mary Beth
dc.date2014-05-27T11:22:25Z
dc.date2016-10-25T18:23:37Z
dc.date2014-05-27T11:22:25Z
dc.date2016-10-25T18:23:37Z
dc.date2007-03-01
dc.date.accessioned2017-04-06T01:23:54Z
dc.date.available2017-04-06T01:23:54Z
dc.identifierJournal of Immune Based Therapies and Vaccines, v. 5.
dc.identifier1476-8518
dc.identifierhttp://hdl.handle.net/11449/69542
dc.identifierhttp://acervodigital.unesp.br/handle/11449/69542
dc.identifier10.1186/1476-8518-5-1
dc.identifier2-s2.0-33847190253.pdf
dc.identifier2-s2.0-33847190253
dc.identifierhttp://dx.doi.org/10.1186/1476-8518-5-1
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/890772
dc.descriptionBackground: Cytosine-phosphate-guanosine oligodeoxynucleotide (CpG-ODN) has been used successfully to induce immune responses against viral and intracellular organisms in mammals. The main objective of this study was to test the effect of CpG-ODN on antigen presenting cells of young foals. Methods: Peripheral blood monocytes of foals (n = 7) were isolated in the first day of life and monthly thereafter up to 3 months of life. Adult horse (n = 7) monocytes were isolated and tested once for comparison. Isolated monocytes were stimulated with IL-4 and GM-CSF (to obtain dendritic cells, DC) or not stimulated (to obtain macrophages). Macrophages and DCs were stimulated for 14-16 hours with either CpG-ODN, LPS or not stimulated. The stimulated and non-stimulated cells were tested for cell surface markers (CD86 and MHC class II) using flow cytometry, mRNA expression of cytokines (IL-12, IFNα, IL-10) and TLR-9 using real time quantitative RT-PCR, and for the activation of the transcription factor NF-κB p65 using a chemiluminescence assay. Results: The median fluorescence of the MHC class II molecule in non-stimulated foal macrophages and DCs at birth were 12.5 times and 11.2 times inferior, respectively, than adult horse cells (p = 0.009). That difference subsided at 3 months of life (p = 0.3). The expression of the CD86 co-stimulatory molecule was comparable in adult horse and foal macrophages and DCs, independent of treatment. CpG-ODN stimulation induced IL-12p40 (53 times) and IFNα (23 times) mRNA expression in CpG-ODN-treated adult horse DCs (p = 0.078), but not macrophages, in comparison to non-stimulated cells. In contrast, foal APCs did not respond to CpG-ODN stimulation with increased cytokine mRNA expression up to 3 months of age. TLR-9 mRNA expression and NF-kB activation (NF-kB p65) in foal DCs and macrophages were comparable (p > 0.05) to adult horse cells. Conclusion: CpG-ODN treatment did not induce specific maturation and cytokine expression in foal macrophages and DCs. Nevertheless, adult horse DCs, but not macrophages, increased their expression of IL-12 and IFNα cytokines upon CpG-ODN stimulation. Importantly, foals presented an age-dependent limitation in the expression of MHC class II in macrophages and DCs, independent of treatment. © 2007 Flaminio et al; licensee BioMed Central Ltd.
dc.languageeng
dc.relationJournal of Immune Based Therapies and Vaccines
dc.rightsinfo:eu-repo/semantics/openAccess
dc.subjectalpha interferon
dc.subjectCD86 antigen
dc.subjectcell surface marker
dc.subjectCpG oligodeoxynucleotide
dc.subjectcytokine
dc.subjectgranulocyte macrophage colony stimulating factor
dc.subjectimmunoglobulin enhancer binding protein
dc.subjectinterleukin 10
dc.subjectinterleukin 12
dc.subjectinterleukin 4
dc.subjectlipopolysaccharide
dc.subjectmajor histocompatibility antigen class 2
dc.subjectmessenger RNA
dc.subjecttoll like receptor 9
dc.subjectanimal cell
dc.subjectantigen presenting cell
dc.subjectcell isolation
dc.subjectcell stimulation
dc.subjectchemoluminescence
dc.subjectcontrolled study
dc.subjectdendritic cell
dc.subjectflow cytometry
dc.subjectfoal
dc.subjectgene expression
dc.subjecthorse
dc.subjectmacrophage
dc.subjectmonocyte
dc.subjectnonhuman
dc.subjectreverse transcription polymerase chain reaction
dc.titleThe effect of CpG-ODN on antigen presenting cells of the foal
dc.typeOtro


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