Cardiovascular responses produced by central injection of hydrogen peroxide in conscious rats

dc.contributorUniversidade Estadual Paulista (UNESP)
dc.creatorMáximo Cardoso, Leonardo
dc.creatorde Almeida Colombari, Débora Simões
dc.creatorVanderlei Menani, José
dc.creatorAlves Chianca Jr., Deoclécio
dc.creatorColombari, Eduardo
dc.date2014-05-27T11:22:21Z
dc.date2016-10-25T18:23:22Z
dc.date2014-05-27T11:22:21Z
dc.date2016-10-25T18:23:22Z
dc.date2006-12-11
dc.date.accessioned2017-04-06T01:23:01Z
dc.date.available2017-04-06T01:23:01Z
dc.identifierBrain Research Bulletin, v. 71, n. 1-3, p. 37-44, 2006.
dc.identifier0361-9230
dc.identifierhttp://hdl.handle.net/11449/69419
dc.identifierhttp://acervodigital.unesp.br/handle/11449/69419
dc.identifier10.1016/j.brainresbull.2006.07.013
dc.identifier2-s2.0-33750969390
dc.identifierhttp://dx.doi.org/10.1016/j.brainresbull.2006.07.013
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/890671
dc.descriptionReactive oxygen species (ROS) have been shown to modulate neuronal synaptic transmission and may play a role on the autonomic control of the cardiovascular system. In this study we investigated the effects produced by hydrogen peroxide (H 2O 2) injected alone or combined with the anti-oxidant agent N-acetil-l-cysteine (NAC) or catalase into the fourth brain ventricle (4th V) on mean arterial pressure and heart rate of conscious rats. Moreover the involvement of the autonomic nervous system on the cardiovascular responses to H 2O 2 into the 4th V was also investigated. Male Holtzman rats (280-320 g) with a stainless steel cannula implanted into the 4th V and polyethylene cannulas inserted into the femoral artery and vein were used. Injections of H 2O 2 (0.5, 1.0 and 1.5 μmol/0.2 μL, n = 6) into the 4th V produced transient (for 10 min) dose-dependent pressor responses. The 1.0 and 1.5 μmol doses of H 2O 2 also produced a long lasting bradycardia (at least 24 h with the high dose of H 2O 2). Prior injection of N-acetyl-l-cysteine (250 nmol/1 μL/rat) into the 4th V blockade the pressor response and attenuated the bradycardic response to H 2O 2 (1 μmol/0.5 μL/rat, n = 7) into the 4th V. Intravenous (i.v.) atropine methyl bromide (1.0 mg/kg, n = 11) abolished the bradycardia but did not affect the pressor response to H 2O 2. Prazosin hydrochloride (1.0 mg/kg, n = 6) i.v. abolished the pressor response but did not affect the bradycardia. The increase in the catalase activity (500 UEA/1 μL/rat injected into the 4th V) also abolished both, pressor and bradycardic responses to H 2O 2. The results suggest that increased ROS availability into 4th V simultaneously activate sympathetic and parasympathetic outflow inducing pressor and bradycardic responses. © 2006 Elsevier Inc. All rights reserved.
dc.languageeng
dc.relationBrain Research Bulletin
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.subjectAnti-oxidant
dc.subjectBlood pressure
dc.subjectHeart rate
dc.subjectHydrogen peroxide
dc.subjectMethyl atropine
dc.subjectN-Acetyl-l-cysteine
dc.subjectPrazosin
dc.subjectAnimals
dc.subjectAntihypertensive Agents
dc.subjectAntioxidants
dc.subjectAutonomic Nervous System Diseases
dc.subjectBlood Pressure
dc.subjectBradycardia
dc.subjectCardiovascular Physiology
dc.subjectCatalase
dc.subjectDisease Models, Animal
dc.subjectDose-Response Relationship, Drug
dc.subjectDrug Interactions
dc.subjectHydrogen Peroxide
dc.subjectHypertension
dc.subjectMale
dc.subjectOxidants
dc.subjectParasympathetic Nervous System
dc.subjectParasympatholytics
dc.subjectRats
dc.subjectRats, Sprague-Dawley
dc.subjectReactive Oxygen Species
dc.subjectSympathetic Nervous System
dc.titleCardiovascular responses produced by central injection of hydrogen peroxide in conscious rats
dc.typeOtro


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