Production of monoclonal antibodies against Streptococcus mutans antigens

dc.contributorUniversidade Estadual Paulista (UNESP)
dc.creatorVictor Canettieri, Antonio Carlos
dc.creatorKretchetoff, Fujiko Yamasiro
dc.creatorKoga Ito, Cristiane Yumi
dc.creatorMoreira, Daniella
dc.creatorCondino Fujarra, Fabio José
dc.creatorUnterkircher, Carmelinda Schmidt
dc.date2014-05-27T11:21:59Z
dc.date2016-10-25T18:22:45Z
dc.date2014-05-27T11:21:59Z
dc.date2016-10-25T18:22:45Z
dc.date2006-10-01
dc.date.accessioned2017-04-06T01:20:45Z
dc.date.available2017-04-06T01:20:45Z
dc.identifierBrazilian Oral Research, v. 20, n. 4, p. 297-302, 2006.
dc.identifier1806-8324
dc.identifier1807-3107
dc.identifierhttp://hdl.handle.net/11449/69140
dc.identifierhttp://acervodigital.unesp.br/handle/11449/69140
dc.identifier10.1590/S1806-83242006000400003
dc.identifierS1806-83242006000400003
dc.identifier2-s2.0-33846846708.pdf
dc.identifier2-s2.0-33846846708
dc.identifierhttp://dx.doi.org/10.1590/S1806-83242006000400003
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/890415
dc.descriptionSeveral studies have been conducted in the last decades aiming to obtain an anti-canies vaccine, however some studies have demonstrated cross reactivity between Streptococcus mutans surface antigens and the human cardiac tissue. In this work, the reactivity of five anti-Streptococcus mutans monoclonal antibodies (MoAb) (24A, 56G, G8, E8 and F6) was tested against oral streptococci, cardiac antigens and skeletal and cardiac myosins, aiming to evaluate the specificity of these MoAb. The hybrid producers of immunoglobulins of the IgG 2b class were cloned by limit dilution and expanded in vivo. MoAb were tested by ELISA. The hybrid 24A reacted with S. mutans CCT 1910, S. salivarius CCT 0365 and S. pyogenes T23. No reactivity difference was observed among the tested species. Cross reactivity with heart and cardiac myosin was not confirmed and only reaction with myosin of skeletal muscle was observed (p = 0.0381). The hybrid 56G reacted with all the tested microorganisms and there was statistically significant difference between S. mutans and S. pyogenes T23 (p < 0.001). This hybrid also reacted with myosin of skeletal muscle (p = 0.0095). C8, E8 and F6 presented low reactivity against oral streptococci strains and no reactivity against cardiac antigens. The data of this study showed that the 24A and 56G anti-S. mutans MoAb presented reactivity with S. pyogenes and S. salivarius, reinforcing the occurrence of common antigens between these species. The tested MoAb presented low cross-reactivity with myosin of skeletal muscle, but anti-heart activity could not be confirmed.
dc.languageeng
dc.relationBrazilian Oral Research
dc.rightsinfo:eu-repo/semantics/openAccess
dc.subjectAntibodies
dc.subjectMonoclonal
dc.subjectStreptococcus mutans
dc.subjectbacterial antigen
dc.subjectbacterium antibody
dc.subjectimmunoglobulin G
dc.subjectmonoclonal antibody
dc.subjectStreptococcus vaccine
dc.subjectanimal
dc.subjectantibody specificity
dc.subjectBagg albino mouse
dc.subjectbiosynthesis
dc.subjectcell culture
dc.subjectdental caries
dc.subjectdisease model
dc.subjectenzyme linked immunosorbent assay
dc.subjectfemale
dc.subjecthuman
dc.subjectimmunology
dc.subjectmouse
dc.subjectswine
dc.subjectAnimals
dc.subjectAntibodies, Bacterial
dc.subjectAntibodies, Monoclonal
dc.subjectAntibody Specificity
dc.subjectAntigens, Bacterial
dc.subjectCells, Cultured
dc.subjectDental Caries
dc.subjectDisease Models, Animal
dc.subjectEnzyme-Linked Immunosorbent Assay
dc.subjectFemale
dc.subjectHumans
dc.subjectImmunoglobulin G
dc.subjectMice
dc.subjectMice, Inbred BALB C
dc.subjectStreptococcal Vaccines
dc.subjectSwine
dc.titleProduction of monoclonal antibodies against Streptococcus mutans antigens
dc.typeOtro


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