Effect of post-polymerization heat treatments on the cytotoxicity of two denture base acrylic resins

dc.contributorUniversidade Estadual Paulista (UNESP)
dc.creatorJorge, Janaina Habib
dc.creatorGiampaolo, Eunice Teresinha
dc.creatorVergani, Carlos Eduardo
dc.creatorMachado, Ana Lucia
dc.creatorPavarina, Ana Claudia
dc.creatorCarlos, Iracilda Zeppone
dc.date2014-05-27T11:21:57Z
dc.date2016-10-25T18:22:35Z
dc.date2014-05-27T11:21:57Z
dc.date2016-10-25T18:22:35Z
dc.date2006-08-31
dc.date.accessioned2017-04-06T01:20:05Z
dc.date.available2017-04-06T01:20:05Z
dc.identifierJournal of Applied Oral Science, v. 14, n. 3, p. 203-207, 2006.
dc.identifier1678-7757
dc.identifier1678-7765
dc.identifierhttp://hdl.handle.net/11449/69050
dc.identifierhttp://acervodigital.unesp.br/handle/11449/69050
dc.identifier10.1590/S1678-77572006000300011
dc.identifierS1678-77572006000300011
dc.identifier2-s2.0-33747834649.pdf
dc.identifier2-s2.0-33747834649
dc.identifierhttp://dx.doi.org/10.1590/S1678-77572006000300011
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/890344
dc.descriptionIntroduction: Most denture base acrylic resins have polymethylmethacrylate in their composition. Several authors have discussed the polymerization process involved in converting monomer into polymer because adequate polymerization is a crucial factor in optimizing the physical properties and biocompatibility of denture base acrylic resins. To ensure the safety of these materials, in vitro cytotoxicity assays have been developed as preliminary screening tests to evaluate material biocompatibility. 3H-thymidine incorporation test, which measures the number of cells synthesizing DNA, is one of the biological assays suggested for cytotoxicity testing. Aim: The purpose of this study was to investigate, using 3H-thymidine incorporation test, the effect of microwave and water-bath post-polymerization heat treatments on the cytotoxicity of two denture base acrylic resins. Materials and Methods: Nine disc-shaped specimens (10 x 1 mm) of each denture base resin (Lucitone 550 and QC 20) were prepared according to the manufacturers' recommendations and stored in distilled water at 37°C for 48 h. The specimens were assigned to 3 groups: 1) post-polymerization in a microwave oven for 3 min at 500 W; 2) post-polymerization in water-bath at 55°C for 60 min; and 3) without post-polymerization. For preparation of eluates, 3 discs were placed into a sterile glass vial with 9 mL of Eagle's medium and incubated at 37°C for 24 h. The cytotoxic effect of the eluates was evaluated by 3H-thymidine incorporation. Results: The results showed that the components leached from the resins were cytotoxic to L929 cells, except for the specimens heat treated in water bath (p<0.05). Compared to the group with no heat treatment, water-bath decreased the cytotoxicity of the denture base acrylic resins. Conclusion: The in vitro cytotoxicity of the tested denture base materials was not influenced by microwave post-polymerization heat treatment.
dc.languageeng
dc.relationJournal of Applied Oral Science
dc.rightsinfo:eu-repo/semantics/openAccess
dc.subjectCell culture
dc.subjectCytotoxicity
dc.subjectDenture base resins
dc.titleEffect of post-polymerization heat treatments on the cytotoxicity of two denture base acrylic resins
dc.typeOtro


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