Optimization of xylanase biosynthesis by Aspergillus japonicus isolated from a Caatinga area in the Brazilian state of Bahia

dc.contributorUniversidade Estadual Paulista (UNESP)
dc.creatorSimões, Maria Lúcia Garcia
dc.creatorTauk-Tornisielo, Samia Maria
dc.date2014-05-27T11:21:53Z
dc.date2016-10-25T18:22:15Z
dc.date2014-05-27T11:21:53Z
dc.date2016-10-25T18:22:15Z
dc.date2006-06-02
dc.date.accessioned2017-04-06T01:18:58Z
dc.date.available2017-04-06T01:18:58Z
dc.identifierAfrican Journal of Biotechnology, v. 5, n. 11, p. 1135-1141, 2006.
dc.identifier1684-5315
dc.identifierhttp://hdl.handle.net/11449/68912
dc.identifierhttp://acervodigital.unesp.br/handle/11449/68912
dc.identifier10.5897/AJB06.151
dc.identifierWOS:000238090400022
dc.identifier2-s2.0-33745041560.pdf
dc.identifier2-s2.0-33745041560
dc.identifierhttp://dx.doi.org/10.5897/AJB06.151
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/890220
dc.descriptionThe objective of this research was to investigate the potential of xylanase production by Aspergillus japonicus and to determine the effects of cultivation conditions in the process, aiming toward optimization of enzyme production. The best temperature, as well as the best carbon source, for biomass production was determined through an automated turbidimetric method (Bioscreen-C). The enzyme activity of this fungus was separately evaluated in two solid substrates (wheat and soybean bran) and in Vogel medium, adding other carbon sources. Temperature effects, cultivation time, and spore concentrations were also tested. The best temperature for enzyme and biomass production was 25°C; however, the best carbon source for growth (determined by the Bioscreen C) did not turn out to be a good inducer of xylanase production. Maximum xylanase activity was achieved when the fungus was cultivated in wheat bran (without the addition of any other carbon source) using a spore concentration of 1 × 107 spores/mL (25°C, pH 5.0, 120 h). A. japonicus is a good xylanase producer under the conditions presented in these assays. © 2006 Academic Journals.
dc.languageeng
dc.relationAfrican Journal of Biotechnology
dc.rightsinfo:eu-repo/semantics/openAccess
dc.subjectAspergillus japonicus
dc.subjectEnzyme optimization
dc.subjectXylanase activity
dc.subjectcarbon
dc.subjectxylan endo 1,3 beta xylosidase
dc.subjectAspergillus
dc.subjectaspergillus japonicus
dc.subjectbiomass production
dc.subjectbiosynthesis
dc.subjectbran
dc.subjectBrazil
dc.subjectcarbon source
dc.subjectconcentration (parameters)
dc.subjectenzyme activity
dc.subjectenzyme synthesis
dc.subjectfungus isolation
dc.subjectnonhuman
dc.subjectsoybean
dc.subjectspecies cultivation
dc.subjecttemperature
dc.subjectturbidimetry
dc.subjectwheat
dc.subjectFungi
dc.subjectGlycine max
dc.subjectTriticum aestivum
dc.titleOptimization of xylanase biosynthesis by Aspergillus japonicus isolated from a Caatinga area in the Brazilian state of Bahia
dc.typeOtro


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