Study of DNA damage induced by dental bleaching agents in vitro

dc.contributorUniversidade Estadual Paulista (UNESP)
dc.creatorRibeiro, Daniel Araki
dc.creatorMarques, Mariângela Esther Alencar
dc.creatorSalvadori, Daisy Maria Favero
dc.date2014-05-27T11:21:52Z
dc.date2016-10-25T18:22:14Z
dc.date2014-05-27T11:21:52Z
dc.date2016-10-25T18:22:14Z
dc.date2006-06-01
dc.date.accessioned2017-04-06T01:18:54Z
dc.date.available2017-04-06T01:18:54Z
dc.identifierBrazilian Oral Research, v. 20, n. 1, p. 47-51, 2006.
dc.identifier1806-8324
dc.identifier1807-3107
dc.identifierhttp://hdl.handle.net/11449/68903
dc.identifierhttp://acervodigital.unesp.br/handle/11449/68903
dc.identifier10.1590/S1806-83242006000100009
dc.identifierS1806-83242006000100009
dc.identifier2-s2.0-33744787663.pdf
dc.identifier2-s2.0-33744787663
dc.identifierhttp://dx.doi.org/10.1590/S1806-83242006000100009
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/890212
dc.descriptionDental bleaching is a simple and conservative procedure for aesthetic restoration of vital and non-vital discolored teeth. Nevertheless, a number of studies have demonstrated the risk of tissue damage from the contact of these agents with the oral mucosa. In the current study, the genotoxic potential associated with exposure to dental bleaching agents was assessed by the single cell gel (comet) assay in vitro. Chinese hamster ovary (CHO) cells in vitro were exposed to six commercial dental bleaching agents (Clarigel Gold - Dentsply; Whitespeed - Discus Dental; Nite White - Discus Dental; Magic Bleaching - Vigodent; Whiteness HP - FGM and Lase Peroxide - DMC). The results pointed out that all dental bleaching agents tested contributed to DNA damage as depicted by the mean tail moment, being the strongest effect observed with the highest dose of hydrogen peroxide (Whiteness HP and Lase peroxide, at a 35% concentration). On the other hand, Magic Bleaching (Vigodent) induced the lowest level of DNA breakage. Negative and positive controls displayed absence and presence of DNA-damaging, respectively. Taken together, these results suggest that dental bleaching agents may be a factor that increases the level of DNA damage. A higher concentration of hydrogen peroxide produced higher noxious activities in the genome as detected by single cell gel (comet) assay.
dc.languageeng
dc.relationBrazilian Oral Research
dc.rightsinfo:eu-repo/semantics/openAccess
dc.subjectCHO cells
dc.subjectComet assay
dc.subjectMutagenicity tests
dc.subjectTooth bleaching
dc.subjecthydrogen peroxide
dc.subjectoxidizing agent
dc.subjectanimal
dc.subjectCHO cell
dc.subjectcomet assay
dc.subjectCricetulus
dc.subjectdental care
dc.subjectDNA damage
dc.subjectdrug effect
dc.subjecthamster
dc.subjectin vitro study
dc.subjectnonparametric test
dc.subjectAnimals
dc.subjectCHO Cells
dc.subjectComet Assay
dc.subjectCricetinae
dc.subjectDNA Damage
dc.subjectHydrogen Peroxide
dc.subjectOxidants
dc.subjectStatistics, Nonparametric
dc.subjectTooth Bleaching
dc.titleStudy of DNA damage induced by dental bleaching agents in vitro
dc.typeOtro


Este ítem pertenece a la siguiente institución