Toxoplasma gondii: Comparison of a rhoptry-ELISA with IFAT and MAT for antibody detection in sera of experimentally infected pigs

dc.contributorUniversidade Estadual Paulista (UNESP)
dc.creatorGarcia, João Luis
dc.creatorNavarro, Italmar Teodorico
dc.creatorVidotto, Odilon
dc.creatorGennari, Solange Maria
dc.creatorMachado, Rosângela Zacarias
dc.creatorPereira, Ademir Benedito da Luz
dc.creatorSinhorini, Idercio Luiz
dc.date2014-05-27T11:21:52Z
dc.date2016-10-25T18:22:12Z
dc.date2014-05-27T11:21:52Z
dc.date2016-10-25T18:22:12Z
dc.date2006-06-01
dc.date.accessioned2017-04-06T01:18:48Z
dc.date.available2017-04-06T01:18:48Z
dc.identifierExperimental Parasitology, v. 113, n. 2, p. 100-105, 2006.
dc.identifier0014-4894
dc.identifier1090-2449
dc.identifierhttp://hdl.handle.net/11449/68894
dc.identifierhttp://acervodigital.unesp.br/handle/11449/68894
dc.identifier10.1016/j.exppara.2005.12.011
dc.identifier2-s2.0-33745151992
dc.identifierhttp://dx.doi.org/10.1016/j.exppara.2005.12.011
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/890203
dc.descriptionIndirect ELISA and IFAT have been reported to be more sensitive and specific than agglutination tests. However, MAT is cheaper, easier than the others and does not need special equipment. The purpose of this study was to compare an enzyme linked immunosorbent assay using crude rhoptries of Toxoplasma gondii as coating wells (r-ELISA) with indirect fluorescence antibody test (IFAT) and modified agglutination test (MAT) to detect anti-T. gondii antibodies in sera of experimentally infected pigs. Ten mixed breed pigs between 6.5 and 7.5 weeks old were used. All pigs were negative for the presence of T. gondii antibodies by IFAT (titre < 16), r-ELISA (OD < 0.295) and MAT (titre < 16). Animals received 7 × 107 viable tachyzoites of the RH strain by intramuscular (IM) route at day 0. Serum samples were collected at days -6, 0, 7, 14, 21, 28, 35, 42, 50, and 57. IFAT detected anti-T. gondii antibodies earlier than r-ELISA and MAT. The average of antibody levels was higher at day 35 in IFAT (Log10 = 2.9) and in MAT (Log10 = 3.5), and at day 42 in r-ELISA (OD = 0.797). The antibody levels remained high through the 57th day after inoculation in MAT, and there was a decrease tendency in r-ELISA and IFAT. IFAT was used as gold standard and r-ELISA demonstrated a higher prevalence (73.3%), sensitivity (94.3%), negative predictive value (83.3%), and accuracy (95.6%) than MAT. Kappa agreements among tests were calculated, and the best results were shown by r-ELISA × IFAT (κ = 0.88, p < 0.001). Cross-reaction with Sarcocystis miescheriana was investigated in r-ELISA and OD mean was 0.163 ± 0.035 (n = 65). Additionally, none of the animals inoculated with Sarcocystis reacted positively in r-ELISA. Our results indicate that r-ELISA could be a good method for serological detection of T. gondii infection in pigs. © 2005 Elsevier Inc. All rights reserved.
dc.languageeng
dc.relationExperimental Parasitology
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.subjectApicomplexa
dc.subjectELISA
dc.subjectenzyme linked immunosorbent assay
dc.subjectIFAT
dc.subjectindirect fluorescence antibody test
dc.subjectMAT
dc.subjectmodified agglutination test
dc.subjectPig
dc.subjectRhoptry antigens
dc.subjectToxoplasma gondii
dc.subjectToxoplasmosis
dc.subjectantibody
dc.subjectdiagnostic agent
dc.subjectimmunoglobulin G
dc.subjectmembrane protein
dc.subjectprotozoon antibody
dc.subjectROP 1 protein, Toxoplasma gondii
dc.subjectagglutination test
dc.subjectanimal cell
dc.subjectanimal experiment
dc.subjectanimal tissue
dc.subjectantibody blood level
dc.subjectantibody detection
dc.subjectantibody titer
dc.subjectblood sampling
dc.subjectcalculation
dc.subjectcontrolled study
dc.subjectcross reaction
dc.subjectdiagnostic accuracy
dc.subjectdiagnostic value
dc.subjectfluorescent antibody technique
dc.subjectinoculation
dc.subjectintermethod comparison
dc.subjectnonhuman
dc.subjectpriority journal
dc.subjectSarcocystis
dc.subjectserodiagnosis
dc.subjectswine
dc.subjectanimal
dc.subjectanimal parasitosis
dc.subjectbiosynthesis
dc.subjectblood
dc.subjectcomparative study
dc.subjectfemale
dc.subjectimmunology
dc.subjectisolation and purification
dc.subjectmale
dc.subjectmethodology
dc.subjectprediction and forecasting
dc.subjectprevalence
dc.subjectrandomization
dc.subjectsensitivity and specificity
dc.subjecttime
dc.subjectToxoplasma
dc.subjectAnimalia
dc.subjectSarcocystis miescheriana
dc.subjectSus scrofa
dc.subjectAgglutination Tests
dc.subjectAnimals
dc.subjectAntibodies, Protozoan
dc.subjectEnzyme-Linked Immunosorbent Assay
dc.subjectFemale
dc.subjectFluorescent Antibody Technique, Indirect
dc.subjectImmunoglobulin G
dc.subjectMale
dc.subjectMembrane Proteins
dc.subjectPredictive Value of Tests
dc.subjectPrevalence
dc.subjectRandom Allocation
dc.subjectSensitivity and Specificity
dc.subjectSwine
dc.subjectTime Factors
dc.subjectToxoplasmosis, Animal
dc.titleToxoplasma gondii: Comparison of a rhoptry-ELISA with IFAT and MAT for antibody detection in sera of experimentally infected pigs
dc.typeOtro


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