The plant energy-dissipating mitochondrial systems: Depicting the genomic structure and the expression profiles of the gene families of uncoupling protein and alternative oxidase in monocots and dicots

dc.contributorUniversidade Estadual Paulista (UNESP)
dc.creatorBorecký, Jiří
dc.creatorNogueira, Fábio T. S.
dc.creatorDe Oliveira, Kívia A. P.
dc.creatorMaia, Ivan de Godoy
dc.creatorVercesi, Aníbal E.
dc.creatorArruda, Paulo
dc.date2014-05-27T11:21:49Z
dc.date2016-10-25T18:21:58Z
dc.date2014-05-27T11:21:49Z
dc.date2016-10-25T18:21:58Z
dc.date2006-03-01
dc.date.accessioned2017-04-06T01:17:53Z
dc.date.available2017-04-06T01:17:53Z
dc.identifierJournal of Experimental Botany, v. 57, n. 4, p. 849-864, 2006.
dc.identifier0022-0957
dc.identifier1460-2431
dc.identifierhttp://hdl.handle.net/11449/68788
dc.identifierhttp://acervodigital.unesp.br/handle/11449/68788
dc.identifier10.1093/jxb/erj070
dc.identifier2-s2.0-33644897887
dc.identifierhttp://dx.doi.org/10.1093/jxb/erj070
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/890102
dc.descriptionThe simultaneous existence of alternative oxidases and uncoupling proteins in plants has raised the question as to why plants need two energy-dissipating systems with apparently similar physiological functions. A probably complete plant uncoupling protein gene family is described and the expression profiles of this family compared with the multigene family of alternative oxidases in Arabidopsis thaliana and sugarcane (Saccharum sp.) employed as dicot and monocot models, respectively. In total, six uncoupling protein genes, AtPUMP1-6, were recognized within the Arabidopsis genome and five (SsPUMP1-5) in a sugarcane EST database. The recombinant AtPUMP5 protein displayed similar biochemical properties as AtPUMP1. Sugarcane possessed four Arabidopsis AOx1-type orthologues (SsAOx1a-1d); no sugarcane orthologue corresponding to Arabidopsis AOx2-type genes was identified. Phylogenetic and expression analyses suggested that AtAOx1d does not belong to the AOx1-type family but forms a new (AOx3-type) family. Tissue-enriched expression profiling revealed that uncoupling protein genes were expressed more ubiquitously than the alternative oxidase genes. Distinct expression patterns among gene family members were observed between monocots and dicots and during chilling stress. These findings suggest that the members of each energy-dissipating system are subject to different cell or tissue/organ transcriptional regulation. As a result, plants may respond more flexibly to adverse biotic and abiotic conditions, in which oxidative stress is involved. © The Author [2006]. Published by Oxford University Press [on behalf of the Society for Experimental Biology]. All rights reserved.
dc.languageeng
dc.relationJournal of Experimental Botany
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.subjectAlternative oxidase
dc.subjectExpression profile
dc.subjectOxidative stress
dc.subjectUncoupling proteins
dc.subjectGenes
dc.subjectPlants (botany)
dc.subjectProteins
dc.subjectSugar cane
dc.subjectTissue
dc.subjectDicots
dc.subjectMonocots
dc.subjectEnergy dissipation
dc.subjectalternative oxidase
dc.subjectcarrier protein
dc.subjection channel
dc.subjectmembrane protein
dc.subjectmessenger RNA
dc.subjectmitochondrial protein
dc.subjectmitochondrial uncoupling protein
dc.subjectoxidoreductase
dc.subjectvegetable protein
dc.subjectamino acid sequence
dc.subjectArabidopsis
dc.subjectbiology
dc.subjectcold
dc.subjectcomparative study
dc.subjectenzymology
dc.subjectgene expression profiling
dc.subjectgenetics
dc.subjectgenome
dc.subjectmetabolism
dc.subjectmitochondrion
dc.subjectmolecular genetics
dc.subjectmultigene family
dc.subjectphylogeny
dc.subjectphysiology
dc.subjectreverse transcription polymerase chain reaction
dc.subjectsequence alignment
dc.subjectsequence analysis
dc.subjectsugarcane
dc.subjectAmino Acid Sequence
dc.subjectCarrier Proteins
dc.subjectCold
dc.subjectComputational Biology
dc.subjectGene Expression Profiling
dc.subjectGenome, Plant
dc.subjectIon Channels
dc.subjectMembrane Proteins
dc.subjectMitochondria
dc.subjectMitochondrial Proteins
dc.subjectMolecular Sequence Data
dc.subjectMultigene Family
dc.subjectOxidoreductases
dc.subjectPhylogeny
dc.subjectPlant Proteins
dc.subjectReverse Transcriptase Polymerase Chain Reaction
dc.subjectRNA, Messenger
dc.subjectSaccharum
dc.subjectSequence Alignment
dc.subjectSequence Analysis, Protein
dc.subjectEnergy
dc.subjectGenotypes
dc.subjectPlant Tissues
dc.subjectPlants
dc.subjectSugar Cane
dc.subjectArabidopsis thaliana
dc.subjectDicotyledoneae
dc.subjectLiliopsida
dc.subjectSaccharum hybrid cultivar
dc.subjectSaccharum sp.
dc.titleThe plant energy-dissipating mitochondrial systems: Depicting the genomic structure and the expression profiles of the gene families of uncoupling protein and alternative oxidase in monocots and dicots
dc.typeOtro


Este ítem pertenece a la siguiente institución