Production of saccharogenic and dextrinogenic amylases by Rhizomucor pusillus A 13.36

dc.contributorUniversidade Estadual Paulista (UNESP)
dc.creatorSilva, Tony M.
dc.creatorAttili-Angelis, Derlene
dc.creatorAzevedo Carvalho, Ana Flávia
dc.creatorDa Silva, Roberto
dc.creatorBoscolo, Maurício
dc.creatorGomes, Eleni
dc.date2014-05-27T11:21:45Z
dc.date2016-10-25T18:21:41Z
dc.date2014-05-27T11:21:45Z
dc.date2016-10-25T18:21:41Z
dc.date2005-12-01
dc.date.accessioned2017-04-06T01:16:48Z
dc.date.available2017-04-06T01:16:48Z
dc.identifierJournal of Microbiology, v. 43, n. 6, p. 561-568, 2005.
dc.identifier1225-8873
dc.identifierhttp://hdl.handle.net/11449/68660
dc.identifierhttp://acervodigital.unesp.br/handle/11449/68660
dc.identifierWOS:000234356600013
dc.identifier2-s2.0-30744471197.pdf
dc.identifier2-s2.0-30744471197
dc.identifierhttp://www.ncbi.nlm.nih.gov/pubmed/16410774
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/889983
dc.descriptionA newly-isolated thermophilic strain of the zygomycete fungus Rhizomucor pusillus 13.36 produced highly active dextrinogenic and saccharogenic enzymes. Cassava pulp was a good alternative substrate for amylase production. Dextrinogenic and saccharogenic amylases exhibited optimum activities at a pH of 4.0-4.5 and 5.0 respectively and at a temperature of 75°C. The enzymes were highly thermostable, with no detectable loss of saccharogenic or dextrinogenic activity after 1 h and 6 h at 60°C, respectively. The saccharogenic activity was inhibited by Ca2+ while the dextrinogenic was indifferent to this ion. Both activities were inhibited by Fe2+ and Cu2+ Hydrolysis of soluble starch by the crude enzyme yielded 66% glucose, 19.5% maltose, 7.7% maltotriose and 6.6% oligosaccharides. Copyright © 2005, The Microbiological Society of Korea.
dc.languageeng
dc.relationJournal of Microbiology
dc.rightsinfo:eu-repo/semantics/openAccess
dc.subjectAmylase
dc.subjectRhizomucor pusillus
dc.subjectThermostability
dc.subjectamylase
dc.subjectcalcium
dc.subjectcopper
dc.subjectenzyme inhibitor
dc.subjectglucose
dc.subjectiron
dc.subjectmaltose
dc.subjectmaltotriose
dc.subjectoligosaccharide
dc.subjectstarch
dc.subjectsucrose
dc.subjecttrisaccharide
dc.subjectbiosynthesis
dc.subjectcassava
dc.subjectenzyme stability
dc.subjectenzymology
dc.subjectmetabolism
dc.subjectpH
dc.subjectRhizomucor
dc.subjecttemperature
dc.subjecttime
dc.subjectAmylases
dc.subjectCalcium
dc.subjectCopper
dc.subjectEnzyme Inhibitors
dc.subjectEnzyme Stability
dc.subjectGlucose
dc.subjectHydrogen-Ion Concentration
dc.subjectIron
dc.subjectMaltose
dc.subjectManihot
dc.subjectOligosaccharides
dc.subjectStarch
dc.subjectSucrose
dc.subjectTemperature
dc.subjectTime Factors
dc.subjectTrisaccharides
dc.subjectFungi
dc.subjectManihot esculenta
dc.titleProduction of saccharogenic and dextrinogenic amylases by Rhizomucor pusillus A 13.36
dc.typeOtro


Este ítem pertenece a la siguiente institución