Metagenome of a Microbial Community Inhabiting a Metal-Rich Tropical Stream Sediment.

dc.creatorCosta, Patrícia Silva
dc.creatorGuimarães, Mariana de Paula Reis
dc.creatorÁvila, Marcelo de Paula
dc.creatorLeite, Laura Rabelo
dc.creatorAraújo, Flávio Marcos Gomes de
dc.creatorSalim, Anna Christina de Matos
dc.creatorOliveira, Guilherme Correa de
dc.creatorBarbosa, Francisco
dc.creatorSouza, Edmar Chartone
dc.creatorNascimento, Andrea Maria Amaral
dc.date2016-01-28T12:50:41Z
dc.date2016-01-28T12:50:41Z
dc.date2015
dc.date.accessioned2023-09-27T00:16:09Z
dc.date.available2023-09-27T00:16:09Z
dc.identifierCOSTA, Patrícia Silva et al. Metagenome of a Microbial Community Inhabiting a Metal-Rich Tropical Stream Sediment. PLoS One, vol. 10, n. 3, p. e0119465, 2015.
dc.identifier1932-6203
dc.identifier10.1371/journal.pone.0119465.
dc.identifierhttps://www.arca.fiocruz.br/handle/icict/12642
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/8899140
dc.descriptionHere, we describe the metagenome and functional composition of a microbial community in a historically metal-contaminated tropical freshwater stream sediment. The sediment was collected from the Mina Stream located in the Iron Quadrangle (Brazil), one of the world's largest mining regions. Environmental DNA was extracted and was sequenced using SOLiD technology, and a total of 7.9 Gbp was produced. A taxonomic profile that was obtained by comparison to the Greengenes database revealed a complex microbial community with a dominance of Proteobacteria and Parvarcheota. Contigs were recruited by bacterial and archaeal genomes, especially Candidatus Nitrospira defluvii and Nitrosopumilus maritimus, and their presence implicated them in the process of N cycling in the Mina Stream sediment (MSS). Functional reconstruction revealed a large, diverse set of genes for ammonium assimilation and ammonification. These processes have been implicated in the maintenance of the N cycle and the health of the sediment. SEED subsystems functional annotation unveiled a high degree of diversity of metal resistance genes, suggesting that the prokaryotic community is adapted to metal contamination. Furthermore, a high metabolic diversity was detected in the MSS, suggesting that the historical arsenic contamination is no longer affecting the prokaryotic community. These results expand the current knowledge of the microbial taxonomic and functional composition of tropical metal-contaminated freshwater sediments.
dc.formatapplication/pdf
dc.languageeng
dc.publisherPublic Library of Science
dc.rightsopen access
dc.subjectArchaea/classification
dc.subjectArchaea/isolation & purification
dc.subjectEnvironmental Microbiology Metagenome
dc.subjectProteobacteria/genetics
dc.subjectSequence Analysis, RNA
dc.titleMetagenome of a Microbial Community Inhabiting a Metal-Rich Tropical Stream Sediment.
dc.typeArticle


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