Seroprevalence and detection of Trypanosoma cruzi in dogs living in a non-endemic area for Chagas disease in the legal Amazon region, Brazil

dc.creatorCosta, Thaliane França
dc.creatorRocha, Ana Vitória Verde Oliveira
dc.creatorMiranda, Leandro Macedo
dc.creatorLima, Larissa Fernanda Soares
dc.creatorSantos, Fred Luciano Neves
dc.creatorSilva, Angelo Antônio Oliveira
dc.creatorSouza, Fernando Almeida
dc.creatorSevá, Anaiá da Paixão
dc.creatorCabral, Aline Diniz
dc.creatorSperança, Márcia Aparecida
dc.creatorCosta, Francisco Borges
dc.creatorNogueira, Rita de Maria Seabra
dc.creatorCosta, Andréa Pereira da
dc.date2021-12-06T12:51:40Z
dc.date2021-12-06T12:51:40Z
dc.date2021
dc.date.accessioned2023-09-27T00:16:06Z
dc.date.available2023-09-27T00:16:06Z
dc.identifierCOSTA, Thaliane França et al. Seroprevalence and detection of Trypanosoma cruzi in dogs living in a non-endemic area for Chagas disease in the legal Amazon region, Brazil. Veterinary Parasitology, v. 26, p. 1-8, Dec. 2021.
dc.identifier0304-4017
dc.identifierhttps://www.arca.fiocruz.br/handle/icict/50177
dc.identifier10.1016/j.vprsr.2021.100648
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/8899132
dc.descriptionSuperior/CAPES (finance cod 001) Conselho Nacional de Desenvolvimento Científico e Tecnol´ogico/CNPq (grant number: 420574/2018-2) and Fundaç˜ao de Amparo `a Pesquisa e ao Desenvolvimento Científico e Tecnol´ogico do Maranh˜ao (grant number 05347/18, BEST-BR-02342/19, BEST-BR-02343/19, BEST-02344/19, BINST-02353/19, BINST-02356/19, BINST-02355/19).
dc.descriptionTrypanosoma cruzi, the etiological agent for Chagas disease, is widely distributed in the Americas. Its hosts are humans and wild and domestic mammals, and its vectors are triatomine insects. Studies have indicated that domestic dogs are sentinel animals in the epidemiology of Chagas disease in endemic regions, including states in the Legal Amazon region of Brazil. In São Luís, the capital of Maranhão, a non-endemic state, the existence of a domestic cycle involving domestic rats has been proven, along with a wild cycle maintained by didelphids. However, no studies on T. cruzi infection in domestic animals in this locality have been conducted. The aim of this study was to investigate occurrence of T. cruzi in dogs living in the Itaqui Bacanga district of São Luís, Maranhão, by means of serological and molecular tests. Blood samples were obtained from 330 dogs and structured epidemiological questionnaires were applied to their keepers. These samples were used in the indirect immunofluorescent antibody test (IFAT), enzyme-linked immunosorbent assay (ELISA) and polymerase chain reaction (PCR). Fisher’s exact test was used for statistical calculations with the aim of identifying risk factors. Out of the 330 animals, 105 (31.8%) were reactive in IFAT, 46 (13.0%) in ELISA and 20 (6.0%) in both serological tests. The results were not significant (p > 0.05) when submitted to statistical analysis for the studied variables. From PCR, 58 samples (17.5%) were found to be positive and, of these, one (0.3%) showed similarity to T. cruzi after sequencing. These data demonstrate that dogs were exposed to and infected by T. cruzi. Thus, they can be considered sentinel animals for Chagas disease in the locality studied, which signals that there is a need for epidemiological surveillance actions.
dc.formatapplication/pdf
dc.languageeng
dc.publisherElsevier
dc.rightsrestricted access
dc.subjectDoença de Chagas
dc.subjectHospedeiro
dc.subjectVigilância sentinela
dc.subjectAmazonas
dc.subjectTrypanosoma cruzi
dc.subjectChagas disease
dc.subjectDomestic host
dc.subjectSentinel
dc.subjectAmazon
dc.titleSeroprevalence and detection of Trypanosoma cruzi in dogs living in a non-endemic area for Chagas disease in the legal Amazon region, Brazil
dc.typeArticle


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