Development of conventional and real-time multiplex PCR-based assays for estimation of natural infection rates and Trypanosoma cruzi load in triatomine vectors

dc.creatorMoreira, Otacilio C.
dc.creatorVerly, Thaiane
dc.creatorAraujo, Paula Finamore
dc.creatorGomes, Suzete A. O.
dc.creatorLopes, Catarina M.
dc.creatorSousa, Danielle M. de
dc.creatorAzevedo, Lívia R.
dc.creatorMota, Fabio .F. da
dc.creatord`Avila-Levy, Claudia M.
dc.creatorSantos-Mallet, Jacenir R.
dc.creatorBritto, Constança
dc.date2017-11-30T16:07:33Z
dc.date2017-11-30T16:07:33Z
dc.date2017
dc.date.accessioned2023-09-27T00:15:59Z
dc.date.available2023-09-27T00:15:59Z
dc.identifierMOREIRA, Otacílio C.; et al. Development of conventional and real-time multiplex PCR-based assays for estimation of natural infection rates and Trypanosoma cruzi load in triatomine vectors. Parasites & Vectors, v.10:404, 14p, 2017.
dc.identifier1756-3305
dc.identifierhttps://www.arca.fiocruz.br/handle/icict/23480
dc.identifier10.1186/s13071-017-2343-x
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/8899119
dc.descriptionChagas disease is a complex anthropozoonosis with distinct domestic and sylvatic mammal species acting as potential reservoirs. The diversity of vector species and their habitats are among the factors that hinder the control of the disease. Control programs periodically monitor the prevalence of T. cruzi infection in insect bugs through microscopical observation of diluted feces. However, microscopy presents limited sensitivity in samples with low parasite numbers, difficulties in examining all evolutionary stages of the insect and may in turn be limited to differentiate T. cruzi from other morphologically similar trypanosomatids. Here, we report two highly sensitive and accurate methodologies to infer T. cruzi infection rates and to quantify parasite load in the gut of field-collected triatomines.
dc.formatapplication/pdf
dc.languageeng
dc.publisherBioMed Central
dc.rightsopen access
dc.subjectTrypanosoma cruzi
dc.subjectReação em Cadeia da Polimerase Multiplex
dc.subjectReação em Cadeia da Polimerase em Tempo Real
dc.subjectTriatominae
dc.subjectCarga Parasitária
dc.subjectInfecção natural
dc.subjectTrypanosoma cruzi
dc.subjectMultiplex PCR
dc.subjectReal-time qPCR
dc.subjectTriatomines
dc.subjectNatural infection
dc.subjectParasite load
dc.titleDevelopment of conventional and real-time multiplex PCR-based assays for estimation of natural infection rates and Trypanosoma cruzi load in triatomine vectors
dc.typeArticle


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