Identification of a new circulating recombinant form of human immunodeficiency virus type 1, CRF124_cpx involving subtypes A, G, H, and CRF27_cpx in Angola

dc.creatorSilva, Rayana Katylin Mendes da
dc.creatorMorais, Joana
dc.creatorFoley, Brian Thomas
dc.creatorBello, Gonzalo
dc.creatorMorgado, Mariza Gonçalves
dc.creatorGuimarães, Monick Lindenmeyer
dc.date2022-11-08T18:33:37Z
dc.date2022-11-08T18:33:37Z
dc.date2022
dc.date.accessioned2023-09-27T00:15:40Z
dc.date.available2023-09-27T00:15:40Z
dc.identifierSILVA, Rayana Katylin Mendes da et al. Identification of a new circulating recombinant form of human immunodeficiency virus type 1, CRF124_cpx involving subtypes A, G, H, and CRF27_cpx in Angola. Frontiers in Microbiology, v. 13, 992640, p. 1-10, Oct. 2022.
dc.identifier1664-302X
dc.identifierhttps://www.arca.fiocruz.br/handle/icict/55539
dc.identifier10.3389/fmicb.2022.992640
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/8899061
dc.descriptionAngola, located in Central Africa, has around 320,000 (270,000–380,000) people living with human immunodeficiency virus (HIV)/AIDS, equivalent to 1% of the country’s population at the end of 2021. A previous study conducted in 2012, using Angolan samples collected between 2008 and 2010 revealed a high prevalence of HIV-1 recombinants, around 42% of sequences, with 21% showing the same UH profile in partial pol region which were grouped into a monophyletic cluster with high bootstrap support. Thus, the objective of the present work was to obtain complete genomes of those sequences and characterize them, aiming at a description of a new circulating recombinant form (CRF). Whole blood from nine HIV-1 UH pol-infected individuals had their genomic DNA extracted, and nested PCR was used to amplify seven overlapping fragments targeting the full-length HIV-1 genome. The final classification was based on maximum likelihood trees, and recombination analyses were performed using a bootscan from the Simplot program. BLAST and Los Alamos Database inspections were used to search other similar H-like pol sequences. Complete genome amplification was possible for three samples, partial genomes were obtained for the other three, and only pol was available for the remaining three sequences. Bootscan analysis of the two whole-genome and three partial genome sequences retrieved from people living with HIV/AIDS (PLHIVA) without epidemiological linkage showed the same complex recombination profile involving HIV-1 subtypes A/G/H/CRF27_ cpx, with a total of six recombinant breakpoints, aiming to classify a new HIV-1 CRF124_cpx. We found no other full-length HIV-1 genomes with the same mosaic profile; however, we identified 33 partial pol sequences, mainly sampled from Angola between 2001 to 2019, with the same H-like profile. Bayesian analysis of H and H-like pol sequences indicates that CRF124_cpx probably originated in Angola at mid-1970s, indicating that this CRF has been circulating in the country for a long time. In summary, our study describes a new CRF circulating principally in Angola and highlights the importance of continuing molecular surveillance studies, especially in countries with high molecular diversity of HIV.
dc.formatapplication/pdf
dc.languageeng
dc.publisherFrontiers Media
dc.rightsopen access
dc.subjectHIV-1
dc.subjectDiversidade
dc.subjectCRF124_cpx
dc.subjectVigilância
dc.subjectAngola
dc.subjectHIV-1
dc.subjectDiversity
dc.subjectCRF124_cpx
dc.subjectSurveillance
dc.subjectAngola
dc.titleIdentification of a new circulating recombinant form of human immunodeficiency virus type 1, CRF124_cpx involving subtypes A, G, H, and CRF27_cpx in Angola
dc.typeArticle


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