Effects of Passovia ovata Mistletoe on Pro-Inflammatory Markers In Vitro and In Vivo

dc.creatorMagalhães, Isadora de Fátima Braga
dc.creatorFigueirêdo, Ana Letícia Marinho
dc.creatorSilva, Elizeu Mendes da
dc.creatorMiranda, Adryan Adam Batalha de
dc.creatorRocha, Cláudia Quintino da
dc.creatorCalabrese, Katia da Silva
dc.creatorSouza, Fernando Almeida
dc.creatorSilva, Ana Lúcia Abreu
dc.date2023-05-26T13:25:52Z
dc.date2023-05-26T13:25:52Z
dc.date2023
dc.date.accessioned2023-09-27T00:15:37Z
dc.date.available2023-09-27T00:15:37Z
dc.identifierMAGALHÃES, Isadora de Fátima Braga et al. Effects of Passovia ovata Mistletoe on Pro-Inflammatory Markers In Vitro and In Vivo. Plants, v. 12, 1814, p. 1 - 15, Apr. 2023.
dc.identifier2223-7747
dc.identifierhttps://www.arca.fiocruz.br/handle/icict/58741
dc.identifier10.3390/ plants12091814
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/8899053
dc.descriptionNew agents that can suppress inflammatory responses are being sought, since chronic inflammation is associated with several pathologies. This work aims to elucidate phytochemicals from the hydroethanolic extract of mistletoe Passovia ovata (POH) and its anti-inflammatory potential. POH is submitted to HPLC-UV, qualitative analysis of chemical constituents, and flavonoid quantification. Cytotoxicity is evaluated in RAW264.7 macrophages by MTT. LPS-stimulated RAW264.7 cells are treated with POH and, after 48 h, the nitrite and cytokine levels are quantified. BALB/c mice are treated by gavage with POH and stimulated with -carrageenan to induce paw oedema or peritonitis. POH yield is 25% with anthraquinones, tannins, anthocyanins, anthocyanidins, flavonols, catechins and flavanones present and flavonoid content of 4.44 0.157 mg QE/g dry weight. POH exhibits low cytotoxicity and significantly reduced (p < 0.01) nitrite, IL-1 , IL-6, and TNF- quantification at 500 g/mL. POH at 500 mg/kg prevents paw edema increase and also reduces inflammatory infiltrate and mast cells in the footpad. In the peritonitis model, POH does not influence cytokines levels or cell counts. Overall, POH demonstrates a high concentration of flavonoids and prominent effects in the reduction in pro-inflammatory markers in vitro and in the inhibition of paw oedema.
dc.formatapplication/pdf
dc.languageeng
dc.publisherMDPI
dc.rightsopen access
dc.subjectVisco
dc.subjectInflamação
dc.subjectMacrófago
dc.subjectCitocina
dc.subjectMastro
dc.subjectMistletoe
dc.subjectInflammation
dc.subjectMacrophage
dc.subjectCytokine
dc.subjectMast cell
dc.titleEffects of Passovia ovata Mistletoe on Pro-Inflammatory Markers In Vitro and In Vivo
dc.typeArticle


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