Eucalyptus ESTs associated with resistance to herbicide inhibitors of aromatic and branched-chain amino acid synthesis

dc.contributorUniversidade Estadual Paulista (UNESP)
dc.creatorVelini, Edivaldo Domingues
dc.creatorTrindade, Maria Lúcia Bueno
dc.creatorAlves, Elza
dc.creatorCatâneo, Ana Catarina
dc.creatorMarino, Celso Luis
dc.creatorde Godoy Maia, Ivan
dc.creatorMori, Edson Seizo
dc.creatorFurtado, Edson Luiz
dc.creatorGuerrini, Iraê Amaral
dc.creatorWilcken, Carlos Frederico
dc.date2014-05-27T11:21:43Z
dc.date2016-10-25T18:21:30Z
dc.date2014-05-27T11:21:43Z
dc.date2016-10-25T18:21:30Z
dc.date2005-12-01
dc.date.accessioned2017-04-06T01:16:05Z
dc.date.available2017-04-06T01:16:05Z
dc.identifierGenetics and Molecular Biology, v. 28, n. 3 SUPPL., p. 575-581, 2005.
dc.identifier1415-4757
dc.identifier1678-4685
dc.identifierhttp://hdl.handle.net/11449/68574
dc.identifierhttp://acervodigital.unesp.br/handle/11449/68574
dc.identifier10.1590/S1415-47572005000400012
dc.identifierS1415-47572005000400012
dc.identifierWOS:000204389800012
dc.identifier2-s2.0-30844471131.pdf
dc.identifier2-s2.0-30844471131
dc.identifierhttp://dx.doi.org/10.1590/S1415-47572005000400012
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/889904
dc.descriptionHerbicides inhibit enzymatic systems of plants. Acetolactate synthase (ALS, EC = 4.1.3.18) and 5-enolpyruvylshikimate-3-phosphate synthase (EPSPS, EC 2.5.1.19) are key enzymes for herbicide action. Hundreds of compounds inhibit ALS. This enzyme is highly variable, enabling the selective control of weeds in a number of crops. Glyphosate, the only commercial herbicide inhibiting EPSPS is widely used for non-selective control of weeds in many crops. Recently, transgenic crops resistant to glyphosate were developed and have been used by farmers. The aim of this study was the data mining of eucalypt expressed sequence tags (ESTs) in the FORESTs Genome Project database (https://forests.esalq.usp.br) related to these enzymes. Representative amino acid sequences from the NCBI database associated with ALS and EPSPS were blasted with ESTs from the FORESTs database using the tBLASTx option of the blast tool. The best blasting reads and clusters from FORESTs, represented as nucleotide sequences, were blasted back with the NCBI database to evaluate the level of similarity with available sequences from different species. One and seven clusters were identified as showing high similarity with EPSPS and ALS sequences from the literature, respectively. The alignment of EPSPS sequences allowed the identification of conserved regions that can be used to design specific primers for additional sequencings. Copyright by the Brazilian Society of Genetics.
dc.descriptionFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.languageeng
dc.relationGenetics and Molecular Biology
dc.rightsinfo:eu-repo/semantics/openAccess
dc.subjectALS
dc.subjectEPSPS
dc.subjectEucalyptus
dc.subjectHerbicides
dc.subject3 phosphoshikimate 1 carboxyvinyltransferase
dc.subjectacetolactate synthase
dc.subjectaromatic amino acid
dc.subjectbranched chain amino acid
dc.subjectglyphosate
dc.subjectherbicide
dc.subjectagricultural worker
dc.subjectamino acid sequence
dc.subjectamino acid synthesis
dc.subjectcontrolled study
dc.subjectcrop
dc.subjectdata base
dc.subjectenzyme inhibition
dc.subjectexpressed sequence tag
dc.subjectFORESTs Genome Project database
dc.subjectGenBank
dc.subjectgene cluster
dc.subjectherbicide resistance
dc.subjectinformation processing
dc.subjectnonhuman
dc.subjectnucleotide sequence
dc.subjecttransgenic plant
dc.subjectweed control
dc.titleEucalyptus ESTs associated with resistance to herbicide inhibitors of aromatic and branched-chain amino acid synthesis
dc.typeOtro


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