Antimicrobial susceptibility determined by the E test, Löwenstein-Jensen proportion, and DNA sequencing methods among Mycobacterium tuberculosis isolates discrepancies, preliminary results

dc.creatorFreixo, Maria Inês Moura
dc.creatorCaldas, Paulo C. S.
dc.creatorSaid, Abbadi
dc.creatorMartins, Fátima
dc.creatorBrito, Rossana Coimbra
dc.creatorFonsec, Leila de Souza
dc.creatorSaad, Maria Helena Féres
dc.date2020-11-18T20:18:28Z
dc.date2020-11-18T20:18:28Z
dc.date2004
dc.date.accessioned2023-09-27T00:15:26Z
dc.date.available2023-09-27T00:15:26Z
dc.identifierFREIXO, Maria Inês Moura et al. Antimicrobial susceptibility determined by the E test, Löwenstein-Jensen proportion, and DNA sequencing methods among Mycobacterium tuberculosis isolates discrepancies, preliminary results. Memórias do Instituto Oswaldo Cruz, Rio de Janeiro, v. 99, n. 1, p. 107-110, Feb. 2004.
dc.identifier0074-0276
dc.identifierhttps://www.arca.fiocruz.br/handle/icict/44524
dc.identifier10.1590/S0074-02762004000100019
dc.identifier1678-8060
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/8899023
dc.descriptionMycobacterium tuberculosis strains resistant to streptomycin (SM), isoniazid (INH), and/or rifampin (RIF) as determined by the conventional Löwenstein-Jensen proportion method (LJPM) were compared with the E test, a minimum inhibitory concentration susceptibility method. Discrepant isolates were further evaluated by BACTEC and by DNA sequence analyses for mutations in genes most often associated with resistance to these drugs (rpsL, katG, inhA, and rpoB). Preliminary discordant E test results were seen in 75% of isolates resistant to SM and in 11% to INH. Discordance improved for these two drugs (63%) for SM and none for INH when isolates were re-tested but worsened for RIF (30%). Despite good agreement between phenotypic results and sequencing analyses, wild type profiles were detected on resistant strains mainly for SM and INH. It should be aware that susceptible isolates according to molecular methods might contain other mechanisms of resistance. Although reproducibility of the LJPM susceptibility method has been established, variable E test results for some M. tuberculosis isolates poses questions regarding its reproducibility particularly the impact of E test performance which may vary among laboratories despite adherence to recommended protocols. Further studies must be done to enlarge the evaluated samples and looked possible mutations outside of the hot spot sequenced gene among discrepant strains.
dc.formatapplication/pdf
dc.languageeng
dc.publisherFundação Oswaldo Cruz. Instituto Oswaldo Cruz.
dc.rightsopen access
dc.subjectMycobacterium tuberculosis
dc.subjectE test
dc.subjectTestes de Sensibilidade Microbiana
dc.subjectAnálise de Sequência de DNA
dc.subjectMycobacterium tuberculosis
dc.subjectSusceptibility test
dc.subjectDNA sequencing
dc.subjectE test
dc.subjectAnálise de Sequência de DNA
dc.subjectTestes de Sensibilidade Microbiana
dc.titleAntimicrobial susceptibility determined by the E test, Löwenstein-Jensen proportion, and DNA sequencing methods among Mycobacterium tuberculosis isolates discrepancies, preliminary results
dc.typeArticle


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