Cholesterol depletion by methyl-β-cyclodextrin enhances cell proliferation and increases the number of desmin-positive cells in myoblast cultures

dc.creatorPortilho, Débora M.
dc.creatorSoares, Carolina P.
dc.creatorMorrot, Alexandre
dc.creatorThiago, Leandro S.
dc.creatorButler-Browne, Gillian
dc.creatorSavino, Wilson
dc.creatorCosta, Manoel L.
dc.creatorMermelstein, Cláudia
dc.date2017-04-20T21:25:27Z
dc.date2017-04-20T21:25:27Z
dc.date2012
dc.date.accessioned2023-09-27T00:15:11Z
dc.date.available2023-09-27T00:15:11Z
dc.identifierPORTILHO, Débora M. et al. Cholesterol depletion by methyl-β-cyclodextrin enhances cell proliferation and increases the number of desmin-positive cells in myoblast cultures. European Journal of Pharmacology, n.694, p.1-12, 2012.
dc.identifier0014-2999
dc.identifierhttps://www.arca.fiocruz.br/handle/icict/18603
dc.identifier10.1016/j.ejphar.2012.07.035
dc.identifier1879-0712
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/8898980
dc.descriptionSkeletal myogenesis comprises myoblast replication and differentiation into striated multinucleated myotubes. Agents that interfere with myoblast replication are important tools for the understanding of myogenesis. Recently, we showed that cholesterol depletion by methyl-β-cyclodextrin (MCD) enhances the differentiation step in chick-cultured myogenic cells, involving the activation of the Wnt/β-catenin signaling pathway. However, the effects of cholesterol depletion on myoblast replication have not been carefully studied. Here we show that MCD treatment increases cell proliferation in primary chick myogenic cell cultures. Treatment of myogenic cells with the anti-mitotic reagent cytosine arabinoside, immediately following cholesterol depletion, blocks the MCD-induced effects on proliferation. Cholesterol depletion induced an increase in the number of desmin-positive mononucleated cells, and an increase in desmin expression. MCD induces an increase in the expression of the cell cycle regulator p53 and the master switch gene MyoD1. Treatment with BIO, a specific inhibitor of GSK3β, induced effects similar to MCD on cell proliferation; while treatment with Dkk1, a specific inhibitor of the Wnt/β-catenin pathway, neutralized the effects of MCD. These findings indicate that rapid changes in the cholesterol content in cell membranes of myoblasts can induce cell proliferation, possibly by the activation of the Wnt/β-catenin signaling pathway.
dc.description2030-01-01
dc.formatapplication/pdf
dc.languageeng
dc.publisherElsevier
dc.rightsrestricted access
dc.subjectMetil-B-ciclodextrina
dc.subjectColesterol
dc.subjectReplicação de células
dc.subjectDesmin
dc.subjectDiferenciação muscular
dc.subjectDesenvolvimento Muscular 
dc.subjectMethyl-B-cyclodextrin
dc.subjectMyogenesis
dc.subjectCholesterol
dc.subjectCell replication
dc.subjectDesmin
dc.subjectMuscle differentiation
dc.subjectDesenvolvimento Muscular 
dc.titleCholesterol depletion by methyl-β-cyclodextrin enhances cell proliferation and increases the number of desmin-positive cells in myoblast cultures
dc.typeArticle


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