Kappa-casein gene study with molecular markers in female buffaloes (Bubalus bubalis)

dc.contributorUniversidade Estadual Paulista (UNESP)
dc.creatorOtaviano, Antonio Roberto
dc.creatorTonhati, Humberto
dc.creatorDesidério Sena, Janete Aparecida
dc.creatorCerón Muñoz, Mario Fernando
dc.date2014-05-27T11:21:43Z
dc.date2016-10-25T18:21:29Z
dc.date2014-05-27T11:21:43Z
dc.date2016-10-25T18:21:29Z
dc.date2005-12-01
dc.date.accessioned2017-04-06T01:16:01Z
dc.date.available2017-04-06T01:16:01Z
dc.identifierGenetics and Molecular Biology, v. 28, n. 2, p. 237-241, 2005.
dc.identifier1415-4757
dc.identifier1678-4685
dc.identifierhttp://hdl.handle.net/11449/68566
dc.identifierhttp://acervodigital.unesp.br/handle/11449/68566
dc.identifier10.1590/S1415-47572005000200010
dc.identifierS1415-47572005000200010
dc.identifierWOS:000230929200010
dc.identifier2-s2.0-30644468865.pdf
dc.identifier2-s2.0-30644468865
dc.identifierhttp://dx.doi.org/10.1590/S1415-47572005000200010
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/889896
dc.descriptionCaseins comprise make up about 80% of the total protein content of milk and present polymorphism with change in the amino acid sequence. Within this abundance of proteins, kappa-casein is noteworthy, since it has been associated with differences in milk yield, composition and processing. The objective of this study was to observe the existence of polymorphism in the kappa-casein gene in female buffaloes. For this purpose, blood samples from 115 female buffaloes, collected with vacutainer by needle punctionure of the jugular vein, were used. for genomic DNA extraction was done from blood samples. The PCR-RFLP and SSCP techniques demonstrated that the studied animals were monomorphic for the kappa-casein gene. Only allele B was observed in these animals, which was present in homozygosis. Therefore, it was not possible to quantify the gene action on milk yield and its constituents. The monomorphism observed in the population studied would allow the development of a method to identify mixtures of cow and buffalo milk in mozzarella cheese production, especially because, in cattle, the kappa-casein gene is polymorphic. Copyright by the Brazilian Society of Genetics.
dc.languageeng
dc.relationGenetics and Molecular Biology
dc.rightsinfo:eu-repo/semantics/openAccess
dc.subjectBuffaloes
dc.subjectPCR-RFLP
dc.subjectPolymorphism
dc.subjectSSCP
dc.subjectgenomic DNA
dc.subjectkappa casein
dc.subjectmolecular marker
dc.subjectallele
dc.subjectanimal experiment
dc.subjectanimal genetics
dc.subjectblood sampling
dc.subjectbuffalo
dc.subjectcheesemaking
dc.subjectcontrolled study
dc.subjectDNA extraction
dc.subjectfemale
dc.subjectgenetic polymorphism
dc.subjecthomozygosity
dc.subjectjugular vein
dc.subjectmilk production
dc.subjectmilk yield
dc.subjectnonhuman
dc.subjectpolymerase chain reaction
dc.subjectrestriction fragment length polymorphism
dc.subjectsingle strand conformation polymorphism
dc.subjectAnimalia
dc.subjectBos taurus
dc.subjectBubalus
dc.subjectBubalus bubalis
dc.titleKappa-casein gene study with molecular markers in female buffaloes (Bubalus bubalis)
dc.typeOtro


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