Frequency of infection of Lutzomyia phlebotomines with Leishmania braziliensis in a Brazilian endemic area as assessed by pinpoint capture and polymerase chain reaction.

dc.creatorMiranda, José Carlos
dc.creatorReis, Eliana Almeida Gomes
dc.creatorSchriefer, Albert
dc.creatorGonçalves, Marilda de Souza
dc.creatorReis, Mitermayer Galvão dos
dc.creatorCarvalho, Lucas Pedreira de
dc.creatorFernandes, Octavio
dc.creatorBarral Netto, Manoel
dc.creatorBarral, Aldina Maria Prado
dc.date2012-09-06T18:11:12Z
dc.date2012-09-06T18:11:12Z
dc.date2002
dc.date.accessioned2023-09-27T00:14:59Z
dc.date.available2023-09-27T00:14:59Z
dc.identifierMIRANDA, J.C. et al. Frequency of infection of Lutzomyia phlebotomines with Leishmania braziliensis in a Brazilian endemic area as assessed by pinpoint capture and polymerase chain reaction. Memórias do Instituto Oswaldo Cruz, v. 97, n. 2, p. 185-8, mar. 2002.
dc.identifierhttps://www.arca.fiocruz.br/handle/icict/5489
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/8898945
dc.descriptionLeishmania infected of Lutzomyia spp. are rare in endemic areas. We tested the hypothesis that there is clustering of infected vectors by combining pinpoint capture with sensitive L. braziliensis kDNA minicircle specific PCR/dot blot in an endemic area in the State of Bahia. Thirty out of 335 samples (10 to 20 sand flies/sample; total of 4,027 female sand flies) were positive by PCR analysis and dot blot leading to a underestimated overall rate of 0.4% positive phlebotomines. However, 83.3% of the positive samples were contributed by a single sector out of four sectors of the whole studied area. This resulted in a rate of 1.5% Leishmania positive phlebotomines for this sector, far above rates of other sectors. Incidence of American cutaneous leishmaniasis cases for this sector was about twice that for other sectors. Our results show that there is a non-homogeneous distribution of Leishmania-infected vectors. Such a clustering may have implications in control strategies against leishmaniasis, and reinforces the necessity of understanding the ecological and geographical factors involved in leishmanial transmission.
dc.formatapplication/pdf
dc.languageeng
dc.rightsopen access
dc.subjectLutzomyia spp
dc.subjectPhlebotomines
dc.subjectLeishmania braziliensis
dc.subjectkDNA PCR
dc.subjectBrazil
dc.subjectInsetos Vetores/parasitologia
dc.subjectLeishmania braziliensis/isolamento & purificação
dc.subjectPsychodidae/parasitologia
dc.subjectAnimais
dc.subjectDNA de Cinetoplasto
dc.subjectDNA de Protozoário
dc.subjectInsetos Vetores/classificação
dc.subjectLeishmania braziliensis/genética
dc.subjectLeishmaniose Cutânea/transmissão
dc.subjectReação em Cadeia da Polimerase
dc.subjectPsychodidae/classificação
dc.titleFrequency of infection of Lutzomyia phlebotomines with Leishmania braziliensis in a Brazilian endemic area as assessed by pinpoint capture and polymerase chain reaction.
dc.typeArticle


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