Real-time PCR versus conventional PCR for malaria parasite detection in low-grade parasitemia

dc.creatorGama, Bianca E.
dc.creatorPires, Felipe do E. S.Silva
dc.creatorLopes, Mauro N'Kruman R.
dc.creatorCardoso, Maria Angélica B
dc.creatorBritto, Constança
dc.creatorTorres, Kátia L. de Mendonça
dc.creatorLima, Leila
dc.creatorSouza, José Maria de
dc.creatorDaniel-Ribeiro, Cláudio Tadeu
dc.creatorCruz, Maria de Fátima Ferreira da
dc.date2018-08-23T13:45:16Z
dc.date2018-08-23T13:45:16Z
dc.date2007
dc.date.accessioned2023-09-27T00:14:48Z
dc.date.available2023-09-27T00:14:48Z
dc.identifierGAMA, Bianca E. et al. Real-time PCR versus conventional PCR for malaria parasite detection in low-grade parasitemia. Experimental Parasitology, v.116, p.427-432, 2007.
dc.identifier0014-4894
dc.identifierhttps://www.arca.fiocruz.br/handle/icict/28324
dc.identifier10.1016/j.exppara.2007.02.011
dc.identifier1090-2449
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/8898914
dc.descriptionWe have optimized a faster and cheaper real-time PCR and developed a conventional genus specific PCR based on 18S rRNA gene to detect malaria parasites in low-grade parasitemias. Additionally, we compared these PCRs to the OptiMAL-IT test. Since there is no consensus on choice of standard quantitative curve in real-time assays, we decided to investigate the performance of parasite DNA from three different sources: "genome", amplicon and plasmid. The amplicon curve showed the best efficiency in quantifying parasites. Both PCR assays detected 100% of the clinical samples tested; the sensitivity threshold was 0.5 parasite/mul and no PCR positive reaction occurred when malaria parasites were not present. Conversely, if OptiMAL-IT were employed for malaria diagnosis, 30% of false-negative results could be expected. We conclude that PCR assays have potential for detecting malaria parasites in asymptomatic infections, in evaluation of malaria vaccine molecule candidates, for screening blood donors, especially in endemic areas, or even in monitoring malaria therapy.
dc.description2030-01-01
dc.formatapplication/pdf
dc.languageeng
dc.publisherElsevier
dc.rightsrestricted access
dc.subjectMalária
dc.subjectDiagnóstico
dc.subjectReação em Cadeia da Polimerase em Tempo Real
dc.subjectDNA
dc.subjectMalaria
dc.subjectDiagnosis
dc.subjectReal-time PCR
dc.subjectDNA
dc.titleReal-time PCR versus conventional PCR for malaria parasite detection in low-grade parasitemia
dc.typeArticle


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