Vertical Transmission of Zika Virus (Flaviviridae, Flavivirus) in Amazonian Aedes aegypti (Diptera: Culicidae) Delays Egg Hatching and Larval Development of Progeny

dc.creatorChaves, Barbara Aparecida
dc.creatorVieira Junior, Ademir Bentes
dc.creatorSilveira, Karine Renata Dias
dc.creatorPaz, Andreia da Costa
dc.creatorVaz, Evelyn Beatriz da Costa
dc.creatorAraujo, Raphaela Guedes Pereira
dc.creatorRodrigues, Nilton Barnabe
dc.creatorCampolina, Thais Bonifacio
dc.creatorOrfano, Alessandra da Silva
dc.creatorPimenta, Rafael Nacif
dc.creatorVillegas, Luis Eduardo Martinez
dc.creatorMelo, Fabrício Freire de
dc.creatorSilva, Breno de Mello
dc.creatorMonteiro, Wuelton Marcelo
dc.creatorGuerra, Maria das Graças Vale Barbosa
dc.creatorLacerda, Marcus Vinicius Guimarães de
dc.creatorNorris, Douglas Eric
dc.creatorSecundino, Nagila Francinete Costa
dc.creatorPimenta, Paulo Filemon Paolucci
dc.date2020-02-03T18:47:29Z
dc.date2020-02-03T18:47:29Z
dc.date2019
dc.date.accessioned2023-09-27T00:14:27Z
dc.date.available2023-09-27T00:14:27Z
dc.identifierCHAVES, Barbara Aparecida et al. Vertical Transmission of Zika Virus (Flaviviridae, Flavivirus) in Amazonian Aedes aegypti (Diptera: Culicidae) Delays Egg Hatching and Larval Development of Progeny. Journal of Medical Entomology, v. 56, n. 6, p. 1739-1744, 2019.
dc.identifier0022-2585
dc.identifierhttps://www.arca.fiocruz.br/handle/icict/39687
dc.identifier10.1093/jme/tjz110
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/8898854
dc.descriptionZika virus (ZIKV) has emerged as a globally important arbovirus and has been reported from all states of Brazil. The virus is primarily transmitted to humans through the bite of an infective Aedes aegypti (Linnaeus, 1762) or Aedes albopictus (Skuse, 1895). However, it is important to know if ZIKV transmission also occurs from Ae. aegypti through infected eggs to her offspring. Therefore, a ZIKV and dengue virus (DENV) free colony was established from eggs collected in Manaus and maintained until the third-fourth generation in order to conduct ZIKV vertical transmission (VT) experiments which used an infectious bloodmeal as the route of virus exposure. The eggs from ZIKV-infected females were allowed to hatch. The resulting F1 progeny (larvae, pupae, and adults) were quantitative polymerase chain reaction (qPCR) assayed for ZIKV. The viability of ZIKV vertically transmitted to F1 progeny was evaluated by cultivation in C6/36 cells. The effects of ZIKV on immature development of Ae. aegypti was assessed and compared with noninfected mosquitoes. AmazonianAe. aegypti were highly susceptible to ZIKV infection (96.7%), and viable virus passed to their progeny via VT. Moreover, eggs from the ZIKV-infected mosquitoes had a significantly lower hatch rate and the slowest hatching. In addition, the larval development period was slower when compared to noninfected, control mosquitoes. This is the first study to illustrate VT initiated by oral infection of the parental population by using mosquitoes, which originated from the field and a ZIKV strain that is naturally circulating in-country. Additionally, this study suggests that ZIKV present in the Ae. aegypti can modify the mosquito life cycle. The data reported here suggest that VT of ZIKV to progeny from naturally infected females may have a critical epidemiological role in the dissemination and maintenance of the virus circulating in the vector.
dc.description2040-01-01
dc.formatapplication/pdf
dc.languageeng
dc.publisherOxford University Press
dc.rightsrestricted access
dc.subjectAedes aegypti
dc.subjectZika virus
dc.subjectAedes aegypti
dc.subjectZika virus
dc.subjectFitness cost
dc.subjectVertical transmission
dc.titleVertical Transmission of Zika Virus (Flaviviridae, Flavivirus) in Amazonian Aedes aegypti (Diptera: Culicidae) Delays Egg Hatching and Larval Development of Progeny
dc.typeArticle


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