Effectiveness of Household Disinfection Techniques to Remove SARS-Cov-2 from Cloth Masks

dc.creatorMares-Guia, Maria Angélica Monteiro de Mello
dc.creatorPaiva, Anne Aline Pereira de
dc.creatorMello, Vinicius Motta
dc.creatorEller, Cristiane M.
dc.creatorSálvio, Andreza Lemos
dc.creatorNascimento, Felipe F.
dc.creatorSilva, Emanuelle S. R. F.
dc.creatorCampos, Vinicius Tadeu Martins Guerra
dc.creatorMendes, Ygara da Silva
dc.creatorLemos, Elba Regina Sampaio de
dc.creatorSousa Jr., Ivanildo P.
dc.creatorHorta, Marco Aurélio Pereira
dc.date2022-08-17T11:34:55Z
dc.date2022-08-17T11:34:55Z
dc.date2022
dc.date.accessioned2023-09-27T00:14:07Z
dc.date.available2023-09-27T00:14:07Z
dc.identifierMARES-GUIA, Maria Angélica Monteiro de Mello et al. Effectiveness of Household Disinfection Techniques to Remove SARS-Cov-2 from Cloth Masks. Preprints, p. 1 - 16, July 2022.
dc.identifierhttps://www.arca.fiocruz.br/handle/icict/54675
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/8898798
dc.descriptionTo assess the efficacy of washing cloth masks, we simulated SARS-CoV-2 contamination in tricoline fabric and tested decontaminants to reduce viral particles. Viral suspensions using two variants (B.1.1.28 and P.1) were inoculated in these fabrics, and the inactivation kinetics were evaluated after washing with various household disinfection products (Soap powder, Lysoform®, Hypochlorite sodium and 70% Alcohol), rinse numbers, and exposure times. Afterward, the fabrics were washed in sterile water, viral RNA extracted and amplified using RT-qPCR. Finally, viral replication in cell cultures was examined. Our findings show that all biocidal treatments successfully disinfected the tissue tested. Some products showed less reduction in viral loads, Soap powder (1.60 x 104, 1.04 x 103), Soap powder and Lysoform® (1.60 x 104, 1.04 x 103) and Alcohol 70% (1.02 x 103, 5.91 x 101) respectively. However, when sodium hypochlorite was used, this reduction was significantly increased (viral inactivation in 100% of the washes). After the first wash, the reduction of viral particles was greater for the P.1 variant than for the B.1.1.28 variant (W = 51759, p 0.05). In conclusion the sodium hypochlorite role on cloth masks disinfection may also have implications for future health emergencies as well as recommended by WHO.
dc.formatapplication/pdf
dc.languageeng
dc.publisherPreprints.org
dc.rightsopen access
dc.subjectSARS-CoV-2
dc.subjectCOVID-19
dc.subjectReutilize máscaras de tecido
dc.subjectDesinfecção
dc.subjectSARS-CoV-2
dc.subjectCOVID-19
dc.subjectReuse fabric masks
dc.subjectDisinfection
dc.titleEffectiveness of Household Disinfection Techniques to Remove SARS-Cov-2 from Cloth Masks
dc.typePreprint


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