Evaluation of the PBP2 transglycosylase region of Staphylococcus aureus as a target for immunotherapeutic approaches

dc.creatorArgondizzo, Ana Paula Corrêa
dc.creatorSaraiva, Felipe Betoni
dc.creatorAlmeida, Marta
dc.creatorPeres, Amanda Maria Nunes
dc.creatorSenna, José Procópio Moreno
dc.date2021-07-17T19:41:40Z
dc.date2021-07-17T19:41:40Z
dc.date2021
dc.date.accessioned2023-09-27T00:13:57Z
dc.date.available2023-09-27T00:13:57Z
dc.identifierARGONDIZZO, Ana Paula Corrêa et al. Evaluation of the PBP2 transglycosylase region of Staphylococcus aureus as a target for immunotherapeutic approaches. Microbial Pathogenesis, v. 157, 105000, p. 1-8, May 2021.
dc.identifier0882-4010
dc.identifierhttps://www.arca.fiocruz.br/handle/icict/48269
dc.identifier10.1016/j.micpath.2021.105000
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/8898770
dc.descriptionInfections caused by Staphylococcus aureus are increasingly prevalent, and treatment has become more difficult due to the emergence of strains that are resistant to multiple drugs, such as methicillin-resistant Staphylococcus aureus (MRSA). Penicillin-binding proteins (PBPs) are essential enzymes in peptidoglycan biosynthesis. Only found in bacteria, they are an excellent target for the development of bacterial control strategies. S. aureus has 4 PBPs, and only PBP2 has transglycosylation activity, making it a good model to evaluate whether the inactivation of the transglycosylase domain (PBP2t) could lead to bacterial death. (His6)-tagged PBP2t was purified from the E. coli cell lysate using Ni-charged resin, and ELISA and immunoblotting assays demonstrated that PBP2t is immunogenic. Flow cytometry analysis was performed to verify the binding of polyclonal antibodies to the bacterial cell surface. In order to verify the ability to provide protection, immunized mice were challenged with a sublethal dose of MRSA, and the bacterial loads in kidneys and spleen were evaluated. A reduction of 2–2.5 logs was seen in organs from immunized mice compared with the negative controls in two independent assays (p < 0.01). Our results demonstrate that the PBP2t is a promising target for the development of novel antimicrobial strategies, but further testing should be performed to validate the protection conferred by immunization with this protein.
dc.description2023
dc.formatapplication/pdf
dc.languageeng
dc.publisherElsevier
dc.rightsrestricted access
dc.subjectStaphylococcus aureus
dc.subjectPBP2
dc.subjectDomínio transglicosylase de ligação penicilina
dc.subjectProteína 2 (PBP2)
dc.subjectResistência a antibióticos
dc.subjectImunização
dc.subjectStaphylococcus aureus
dc.subjectPBP2
dc.subjectTransglycosylase domain of penicillin binding
dc.subjectprotein 2 (PBP2)
dc.subjectAntibiotic resistance
dc.subjectImmunization
dc.titleEvaluation of the PBP2 transglycosylase region of Staphylococcus aureus as a target for immunotherapeutic approaches
dc.typeArticle


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