Occurrence of uterine carriers for Leptospira interrogans on slaughtered cows

dc.creatorPires, Bruno Cabral
dc.creatorGrapiglia, Junia Berzin
dc.creatorMoreira, Lio
dc.creatorJaeger, Lauren Hubert
dc.creatorCosta, Filipe Anibal Carvalho
dc.creatorLilenbaum, Walter
dc.date2019-02-13T18:03:33Z
dc.date2019-02-13T18:03:33Z
dc.date2018
dc.date.accessioned2023-09-27T00:13:41Z
dc.date.available2023-09-27T00:13:41Z
dc.identifierPIRES, Bruno Cabral; et al. Occurrence of uterine carriers for Leptospira interrogans on slaughtered cows. Microbial Pathogenesis, v.114, p.163-165, Nov. 2018.
dc.identifier0882-4010
dc.identifierhttps://www.arca.fiocruz.br/handle/icict/31614
dc.identifier10.1016/j.micpath.2017.11.056
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/8898725
dc.descriptionReproductive tract is an important site of infection for chronic leptospirosis and cooperate in pathogenesis of reproductive failure, leading to economic losses. Since serology techniques cannot detect chronic carriers, the molecular analysis of clinical samples is an alternative to detect these animals on livestock. The aim of the present study was to perform a retrospective study in order to detect leptospiral uterine carriers in slaughtered cows. Tissue samples were collected from 50 post-pubertal, nonpregnant cows. These samples were fixed in 10% buffered formalin, paraffin-embedded and stored. PCR targeting lipL32 gene and molecular characterization by secY sequence was performed. Leptospiral DNA was identified in 18% (9/50) examined blocks. Two sequences were characterized as L. interrogans. These findings suggest that the presence of infectious leptospires in uterus is associated with the physiopathogenesis of the reproductive failure.
dc.description2030-01-01
dc.formatapplication/pdf
dc.languageeng
dc.publisherElsevier
dc.rightsrestricted access
dc.subjectLeptospirose
dc.subjectÚtero
dc.subjectSequência
dc.subjectTrato genital
dc.subjectLeptospirosis
dc.subjectGenital tract
dc.subjectUterus
dc.subjectSecY
dc.subjectSequencing
dc.titleOccurrence of uterine carriers for Leptospira interrogans on slaughtered cows
dc.typeArticle


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